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Showing: 7781 - 7800 of 9090 results

  1. pQdCas9.luxR(mut)-sggfp

    Plasmid
    #236185
    Purpose
    The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    Quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    Promoter
    Quorum sensing promoter
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    236185-plasmid-map-sequence-id-471055

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  2. pQdCas12a.luxR(mut)-sggfp(C)

    Plasmid
    #236043
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236043-plasmid-map-sequence-id-471082

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  3. pQdCas12a.luxR(mut)-empty

    Plasmid
    #236037
    Purpose
    The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236037-plasmid-map-sequence-id-469909

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  7. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  9. pEF004-sgRNA-shuffle-in

    Plasmid
    #104440
    Purpose
    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Insert
    sgRNA-transRNA transcription by U6 promoter
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    U6 promoter
    Available Since
    Dec. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    104440-plasmid-map-sequence-id-201143

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  11. pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

    Plasmid
    #154829
    Purpose
    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    coEPO
    coEPO
    Use
    Cre/Lox
    Expression
    Mammalian
    Mutation
    Codon-optimized (Cricetulus griseus)
    Promoter
    100RPU.2
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154829-plasmid-map-sequence-id-308567

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  12. pYFAC-CH3

    Plasmid
    #168979
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Available Since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168979-plasmid-map-sequence-id-350204

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  14. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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Showing: 7781 - 7800 of 9090 results