We narrowed to 26,306 results for: Vit

1. mCardinal-Paxillin-22

   Plasmid

   #56171

   Purpose

   Localization: Focal Adhesions, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Paxillin
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pAAV.CMV.VTN.C-HA

   Plasmid

   #154849

   Purpose

   Expresses murine Vitronectin (VTN) protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Vitronectin (Vtn Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

   Plasmid

   #159660

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

   Plasmid

   #159661

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

   Plasmid

   #159659

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-(pos36)GFP-9xGGS-Cre-6xHis

   Plasmid

   #62938

   Purpose

   Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (ps36)GFP-9xGGS-Cre
   

   Tags

   (pos36)GFP and 6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. LightR-iCre-miRFP670

   Plasmid

   #162158

   Purpose

   Expresses LightR-iCre-miRFP670 in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   LightR-iCre-miRFP670
   

   Tags

   NLS and miRFP670

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-evoA1-seBE4max-IRES

   Plasmid

   #174699

   Purpose

   Small-molecule controlled base editing

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

   Insert

   evoA1-seBE4max-IRES
   

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   UGI-E11K

   Promoter

   CMV

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid

    #181857

    Purpose

    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-JNKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid

    #181856

    Purpose

    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AktAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5-rat ERK2-K52R

    Plasmid

    #40813

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    K52R

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pGEMHE-mEGFP-mTrim21ΔRING-Box

    Plasmid

    #105520

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMx/ATP11C-HA

    Plasmid

    #209237

    Purpose

    Mammalian expression of ATP11C

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP11C (ATP11C Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid

    #52494

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-dCas13b-ADAR2DD

    Plasmid

    #190913

    Purpose

    Addgene #103871 with 3x FLAG on N-terminal of dCas13

    Depositor

    Timothy Jarome

    Article

    Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

    Insert

    3X FLAG
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5 HA-GST-Presc-mAkt1 (wt)

    Plasmid

    #48805

    Purpose

    expresses wt Akt1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    Akt1 (Akt1 Mouse)
    

    Tags

    HA-GST-Presc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pYPQ266

    Plasmid

    #164713

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Human HMG-CoA lyase (HL)

    Plasmid

    #52433

    Purpose

    bacterial expression of human HMG-CoA lyase (HL)

    Depositor

    Henry Miziorko

    Article

    Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

    Insert

    HMG CoA lyase (HMGCL Human)
    

    Tags

    His and S

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

20. HES7-NLuc-2A-tdTomato

    Plasmid

    #130932

    Purpose

    Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    HES7 (HES7 Human)
    

    Tags

    NanoLuc and tdTomato

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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