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164,223 results

Showing: 11791 - 11820 of 164223 results

  1. pRDA_118

    Plasmid
    #133459
    Purpose
    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133459-plasmid-map-sequence-id-261403

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  3. pCS2+ H2B-mTagBFP2

    Plasmid
    #99267
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Insert
    mTagBFP2-3XHA
    Use
    Tags
    H2B
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available Since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    99267-plasmid-map-sequence-id-192954

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  4. Epac-S-H188

    Plasmid
    #170349
    Purpose
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170349-plasmid-map-sequence-id-344647

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  6. pCMV-GST-IRP1

    Plasmid
    #216827
    Purpose
    Expresses human IRP1 with a N-terminal GST-tag in mammalian cells.
    Depositor
    Samie Jaffrey
    Insert
    ACO1 (ACO1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216827-plasmid-map-sequence-id-430364

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  8. TFORF2240

    Plasmid
    #141984
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GATA3 (GATA3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    141984-plasmid-map-sequence-id-395925

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  13. PRE-Hsp70BbCas9_1.3

    Plasmid
    #190797
    Purpose
    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190797-plasmid-map-sequence-id-376572

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  19. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73440-plasmid-map-sequence-id-135339

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  20. pEND-349_Cacnes_ori

    Plasmid
    #225604
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225604-plasmid-map-sequence-id-451376

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  21. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available Since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

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  23. TFORF2990

    Plasmid
    #144466
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFE2L1 (NFE2L1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144466-plasmid-map-sequence-id-404205

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  24. SpRY-ABE8e(V106W)

    Plasmid
    #198553
    Purpose
    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198553-plasmid-map-sequence-id-388905

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  27. TFORF0139

    Plasmid
    #141533
    Purpose
    Lentiviral vector for overexpressing the UBTF transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    UBTF (UBTF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141533-plasmid-map-sequence-id-338772

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Showing: 11791 - 11820 of 164223 results