We narrowed to 12,778 results for: NUC
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Plasmid#98697PurposeI-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafishDepositorAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only
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pMyc2ElbLuc,
Plasmid#53244Purposereporter plasmid containing 2 myc cDNA binding sitesDepositorInsertpMyc2ElbLuc (MYC Human)
MutationTo prepare plasmids with one, two, three, and fou…Available SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2
Plasmid#182354PurposeNuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2
Plasmid#182356PurposeNuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
(81) pcDNA3.1-BC2-Nup62-Flag-EPEA
Plasmid#168094PurposeNucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tagsDepositorInsertNup62-Ubc-Flag-EPEA
TagsBC2-Tag, EPEA-tag, and Flag-tagExpressionMammalianMutationP229A, S283T on Nup62 native sequencePromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsub(TA)DD-Venus-NES
Plasmid#84631PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsub(TA)DD-Venus-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationMutated target Thr residue to Ala.PromoterCMVAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDK4-luciferase active (880)
Plasmid#86657PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation867 base pairs including up to coding nucleotide …PromoterCDK5Available SinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/Flag-mKO2-HuR.DelHNS
Plasmid#110373PurposeMammalian expression of mKO2-HuR (DelHNS) with FLAG tag, Flp-In T-Rex systemDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
TagsFLAG and mKO2ExpressionMammalianMutationHNS (HuR nuclear-cytoplasmic shuttling sequence) …PromoterCMVAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES- mCE(K294A)
Plasmid#82464PurposeExpresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)DepositorInsertNES mRNA capping enzyme (Rngtt Mouse, HIV)
TagsmycExpressionMammalianMutationK294APromoterCMVAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-CENPB-N-22
Plasmid#56351PurposeLocalization: Nucleus/Centromeres, Excitation: 530, Emission: 541DepositorInsertCENPB (CENPB Human)
TagsmPapaya1ExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC
Plasmid#67687PurposeUAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassetteDepositorAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP-WAF1-ES
Plasmid#16449DepositorInsertWAF1 stop codon mut (CDKN1A Human)
ExpressionMammalianMutationstop codon at amino acid 49 instead of tryptophan…Available SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
KHBD00681
Plasmid#39726DepositorAvailable SinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-CENPB-N-22
Plasmid#55470PurposeLocalization: Nucleus/Centromeres, Excitation: 462, Emission: 492DepositorInsertCENPB (CENPB Human)
TagsmTFP1ExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(1-114)
Plasmid#99513PurposeTruncated C-terminal AID degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(1-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteMutationinternal inversion of nucleotides 265-312 of the …Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-H3.3-N-14
Plasmid#55490PurposeLocalization: Nucleus/Histones, Excitation: 462, Emission: 492DepositorAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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AAV-eGFP-KASH
Plasmid#248781PurposeAAV construct expressing eGFP-KASH enabling FACS-sorting of extracted nucleiDepositorInserteGFP-KASH
PromoterCbhAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET-hisMBP-rRNaseI
Plasmid#216673PurposeExpresses hisMBP-tagged rat oxidation-resistant RNase inhibitor in E. coli hostsDepositorAvailable SinceNov. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWidom601.CDKN2Bp.601
Plasmid#247019PurposeTwo Widom601 sequences, with a short linker with sequence from the CDKN2B locus between them. For large scale PCR amplification of DNA for nucleosome refolding.DepositorInsertWidom601 sequences and CDKN2B locus
UseSynthetic BiologyAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKMW124_dCas9
Plasmid#244821PurposeMammalian expression of catalytically inactive Cas9 (dCas9)DepositorInsertdCas9 (WT)
UseCRISPRTags3xFLAG-SV40 NLS and Nucleoplasmin NLSExpressionMammalianMutationD10A/H840APromoterCAG, U6Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Split PS Intein gal4-vp16
Plasmid#242030PurposeBlue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus. Split version.DepositorInsertSplit PS Intein gal4-vp16
ExpressionMammalianMutationpNL3.2.NF-KB-RE[NlucP/NF-KB-RE/Hygro]PromoterCMVAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#228557PurposeExpresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertdRfxCas13d
UseLentiviralTagsNLS-HAMutationR239A/H244A/R858A/H863AAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKII-RFP-p2A-DAAO-NES
Plasmid#238918PurposeMammalian expression of DAAO with a nuclear export signal and RFP as a reporter in excitatory neuronsDepositorInsertRFP-p2A-DAAO-NES
UseAAVExpressionMammalianPromoterCaMKIIalfaAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CMV-RFP-p2A-DAAO-NES
Plasmid#238920PurposeExpression of DAAO with a nuclear export signal and RFP as a reporter in mammalian cellsDepositorInsertRFP-p2A-DAAO-NES
UseAAVExpressionMammalianPromoterCMVAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(B)
Plasmid#236041PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(A)
Plasmid#236039PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-MUT-MRE
Plasmid#235148PurposeExpresses the mCherry-based mutated (control) sensor for miR-124. TagBFP2 is expressed as a transduction reporterDepositorInsertmCherry with a mutated miR-124 MRE
UseAAVMutation7 nucleotides of the miR-124 WT MRE mutatedPromoterEFSAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only