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pMS12

Plasmid

#229461

Purpose

Expresses IraD with W64R mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (W64R)

Tags

6xHis

Expression

Bacterial

Mutation

W64R

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF40

Plasmid

#229459

Purpose

Expresses IraD with R70A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R70A)

Tags

6xHis

Expression

Bacterial

Mutation

R70A

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF26

Plasmid

#229458

Purpose

Expresses IraD with Q84A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (Q84A)

Tags

6xHis

Expression

Bacterial

Mutation

Q84A

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF25

Plasmid

#229457

Purpose

Expresses IraD with R67A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R67A)

Tags

6xHis

Expression

Bacterial

Mutation

R67A

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF14

Plasmid

#229455

Purpose

Expresses IraD with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF17

Plasmid

#229454

Purpose

Expresses IraM with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp47

Plasmid

#228212

Purpose

Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

47 (47 Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-AtUBA1-S

Plasmid

#222712

Purpose

Expression AtUBA1-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtUBA1 (UBA1 Mustard Weed)

Tags

S-Tag

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-tdc-1P::AI::acc-1::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225926

Purpose

Expressing ACC-1 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

ACC-1 (acc-1 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225925

Purpose

Expressing LGC-47 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-npr-9P::AI::lgc-47::SL2::tagBFP::unc-54-3'UTR

Plasmid

#225923

Purpose

Expressing LGC-47 in AIB neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

npr-9

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1 mVermillion-T2A-mTurquoise2

Plasmid

#219847

Purpose

equimolar expression of mVermillion and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mVermillion-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 mGarnet2-T2A-mTurquoise2

Plasmid

#219850

Purpose

equimolar expression of mGarnet2 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mGarnet2-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 StayGold-E138D-T2A-mCherry

Plasmid

#219833

Purpose

equimolar expression of Staygold-E138D and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

StayGold-E138D-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-GeneTRAP

Plasmid

#218187

Purpose

To KO and genetrap mouse Vamp2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-Tag-HA

Plasmid

#218188

Purpose

To tag endogenous mouse VAMP2 with HA

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LAMTOR1-APEX2

Plasmid

#215556

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMTOR1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAF146

Plasmid

#222377

Purpose

Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

PDL1

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Vector 1174K

Plasmid

#221017

Purpose

Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)

Depositor

Omar Akbari

Article

Weng et al bioRxiv [Preprint]. 2024 Jul 18:2024.07.

Insert

Engineered dobulesex splicing module (Anopheles gambiae)

Use

Synthetic Biology

Expression

Insect

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1360

Plasmid

#219777

Purpose

pDEF-TurboID-GFPmut2, empty vector for creating C-terminal fusion proteins.

Depositor

Scott Lindner

Article

Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

Insert

TurboID/GFPmut2

Use

Plasmodium, rodent malaria parasites

Promoter

None

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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