We narrowed to 8,596 results for: Set

1. PV504

   Plasmid

   #132343

   Purpose

   CasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   CasA-AT-CBF1 (CBF1 CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
   

   Tags

   AT-CBF1

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLC-Flag-SENP8 sg2R-P2A-Hygro

   Plasmid

   #124300

   Purpose

   Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   SENP8 (SENP8 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   several silent mutations to disrupt sgRNA targeti…

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-30a(+)-C-SH2

   Plasmid

   #111419

   Purpose

   express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

   Insert

   murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-30a(+)-C-SH2+IA

   Plasmid

   #111418

   Purpose

   express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

   Insert

   murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-30a(+)-N-SH2+IA

   Plasmid

   #111273

   Purpose

   express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMGF130

   Plasmid

   #96971

   Purpose

   S. pombe lem2 knockout cassette with hygro resistance gene

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   lem2 (lem2 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   whole ORF deletion and replaced with hygro resist…

   Promoter

   N/A

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMGF169

   Plasmid

   #96996

   Purpose

   ish1 mCherry insertion cassette with G418 resistance gene

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + G418 …

   Promoter

   N/A

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET151-Vps4(101-437)-Hcp1 K321D

   Plasmid

   #89288

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Lys-321 to Asp

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET151-Vps4(101-437)-Hcp1 R325D

   Plasmid

   #89289

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Arg-325 to Asp

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET151-Vps4(101-437)-Hcp1 E322A

    Plasmid

    #87748

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Glu-322 to Ala

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET151-Vps4(101-437)-Hcp1 R325A

    Plasmid

    #87749

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Ala

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET151-Vps4(101-437)-Hcp1 K321A

    Plasmid

    #87747

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Ala

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

    Plasmid

    #83458

    Purpose

    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4127-4303, S4144D

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid

    #69233

    Purpose

    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cbh_v5 AAV-ABE C-terminal

    Plasmid

    #137178

    Purpose

    AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE C-terminal; U6-protospacer
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

    Plasmid

    #233039

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    

    Use

    AAV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiGuide-Hygro-mTagBFP2

    Plasmid

    #99374

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Promoter

    CAG, TetOn

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiGuide-Hygro-eGFP

    Plasmid

    #99375

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-(BbsI)_CBh-Cas9-T2A-BFP

    Plasmid

    #64323

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-EBFP2

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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