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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,682 results for: cell

Showing: 7861 - 7880 of 63682 results

  3. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid
    #176461
    Purpose
    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176461-plasmid-map-sequence-id-346306

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  5. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid
    #198054
    Purpose
    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning
    Depositor
    Lacra Bintu
    Article
    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.
    Inserts
    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G and pEF
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    198054-plasmid-map-sequence-id-391373

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  6. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80066
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80066-plasmid-map-sequence-id-152619

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  9. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80067
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80067-plasmid-map-sequence-id-152621

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  10. pMT-ubb-Avi-Cerulean-Rpl10a

    Plasmid
    #79882
    Purpose
    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    Use
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79882-plasmid-map-sequence-id-149785

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  15. AKT-APEX2-P2A-mKate2

    Plasmid
    #67654
    Purpose
    Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    AKT-PH-APEX2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67654-plasmid-map-sequence-id-116994

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Showing: 7861 - 7880 of 63682 results