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pAAV.Syn.GCaMP6m.WPRE.SV40

Plasmid

#100841

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6m

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVhPOP1_3Flag

Plasmid

#53968

Purpose

Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

POP1 (POP1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mIL-2Rα

Plasmid

#59917

Purpose

Expresses mouse interleukin-2 receptor alpha chain

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pAAV.RMacL3-01.EGFP.BCA5.WPRE

Plasmid

#225685

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-01.EGFP.BCA5

Expression

Mammalian

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP-hDF-deltaTM-pCMV-9

Plasmid

#72586

Purpose

human dysferlin no transmembrane domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

transmembrane domain deleted

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-SomArchon

Plasmid

#126942

Purpose

Expression of SomArchon under CaMKII promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUB6-CDC34-HA (pRKB261)

Plasmid

#99145

Purpose

Mammalian expression construct to express HA-tagged CDC34

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

CDC34 (CDC34 Human)

Tags

HA

Expression

Mammalian

Promoter

pTight

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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UTS2R-DuET

Plasmid

#213386

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

UTS2R (UTS2R Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(Q61L)

Plasmid

#22783

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (Q61L) (RAC1 Human, A. victoria)

Tags

CyPet

Expression

Mammalian

Mutation

Rac1 Q61L.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
STARR-seq luciferase validation vector_mP_empty

Plasmid

#99298

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11A(E186Q)-HA

Plasmid

#209249

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Tags

HA

Expression

Mammalian

Mutation

E186Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadDE-BlastR

Plasmid

#193850

Purpose

Express TadDE in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-hRARβP-FL-luciferase

Plasmid

#135447

Purpose

Human full-length promoter of retinoic acid receptor-beta gene in pGL3-Basic-luciferase plasmid vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

Full-length promoter of human retinoic acid receptor-beta gene

Promoter

Human RARβ promoter

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p205-5'-Switch-ON

Plasmid

#217884

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPBC-G6f

Plasmid

#62811

Purpose

pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6f-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcg 2.0-BFP-circ-arRNA backbone

Plasmid

#213965

Purpose

pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-mCherry-Rheb(C181S)

Plasmid

#192438

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

Lyso-mCherry-Rheb(C181S) (RHEB Human)

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-Rheb(C181S)

Plasmid

#192437

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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NTSR1-DuET

Plasmid

#213356

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

NTSR1 (NTSR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-FLIP1500

Plasmid

#16016

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP (CFLAR Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only