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FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

Plasmid

#194897

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_WT
U6-crRNA-scramble

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK1-P2A-Hygro_Barcode

Plasmid

#170221

Purpose

Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK1 (CDK1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS8/myc-His

Plasmid

#127489

Purpose

Expresses myc-tagged NDUFS8 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS8 (NDUFS8 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_ASAP2-1/1

Plasmid

#91504

Purpose

Protein expression and purification of human SH3 domain construct ASAP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ASAP2-1/1 (ASAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DTYMK

Plasmid

#100546

Purpose

mammalian expression of deoxythymidylate kinase

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

deoxythymidylate kinase (DTYMK Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T2 gp78 G2BR mt / JM26

Plasmid

#13308

Depositor

Allan Weissman

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

Insert

gp78 (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

cDNA corresponds to aa309-643 derived from human …

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

Plasmid

#170217

Purpose

Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CASP8 (CASP8 Human)

Use

Lentiviral

Tags

V5

Mutation

C360A

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

Plasmid

#170225

Purpose

Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

ER (ESR1 Human)

Use

Lentiviral

Tags

V5

Mutation

Y537S

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK6-P2A-Hygro_Barcode

Plasmid

#170224

Purpose

Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911(pL91)

Plasmid

#124390

Purpose

E.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911 wildtype (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-BHLHE41wt-IDR

Plasmid

#145229

Purpose

Expresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

BHLHE41wt-IDR (BHLHE41 Human)

Expression

Mammalian

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4B (205-224) (pGEX2T(TEV))

Plasmid

#80634

Purpose

Residues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4B (CHMP4B Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 205-224

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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GST-ENLYFQC-PNT(46-138)-His6

Plasmid

#42919

Depositor

Barbara Imperiali

Article

Lukovic et al Angew Chem Int Ed Engl. 2009;48(37):6828

Insert

Ets-1 PNT domain (46-138) (Ets1 Mouse)

Tags

6xHis, GST, and TEV protease cleavage site

Expression

Bacterial

Mutation

Contains the PNT domain (Serine 46 through Lysine…

Promoter

Ptac

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
GST-CHMP4A (205-222) E209A (pGEX2T(TEV))

Plasmid

#80640

Purpose

Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Changed Glutamate 209 to alanine

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-mSTING (1-339)

Plasmid

#229740

Purpose

Expresses mouse STING amino acids 1-339 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Tags

None

Expression

Mammalian

Mutation

Contains amino acids 1-339

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP4A 153-222

Plasmid

#108256

Purpose

Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP4A residues 153-222 (CHMP4A Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Contains nonnative Gly-Cys for fluorescent labeli…

Promoter

T7

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human Rab7A (RAB7A Human)

Expression

Mammalian

Promoter

U6

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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GB1-ΔN-CHMP4C(G154C)

Plasmid

#190783

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C)

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CHMP4C(121-233, G154C) (CHMP4C Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

G154C

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-ΔN-CHMP4C(M165C)

Plasmid

#190784

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CHMP4C(121-233, M165C) (CHMP4C Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

M165C

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-ΔN-CHMP4C(S191C)

Plasmid

#180027

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CHMP4C(121-233, S191C) (CHMP4C Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

S191C

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Duet-6xHis-TEV-Nsp8(76-198)

Plasmid

#201024

Purpose

Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

Plasmid

#194890

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA1

Use

Lentiviral

Tags

HA

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

Plasmid

#194895

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-2 targeting the human MECP2 locus.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_Q418A
U6-crRNA2

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

Plasmid

#194889

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 targeting the human MECP2 locus.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_WT
U6-crRNA1

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

Plasmid

#194891

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 targeting the human MECP2 locus.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_Q418A
U6-crRNA1

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

Plasmid

#194896

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-2 targeting the human MECP2 locus.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_R396A
U6-crRNA2

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP

Plasmid

#194888

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-1 and -2 targeting the human MECP2 locus.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_R396A
U6-crRNA1and2

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

Plasmid

#194887

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 and -2 targeting the human MECP2 locus.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_Q418A
U6-crRNA1and2

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

Plasmid

#194893

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-2 targeting the human MECP2 locus.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_WT
U6-crRNA2

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge