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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

Plasmid

#209782

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

sgRNA

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSMP-DNMT1_1

Plasmid

#36382

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

DNMT1 (DNMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pDEST/N1-hEB1-GFP

Plasmid

#27382

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

microtubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pSK+ mBMPR-1B DN

Plasmid

#49530

Purpose

cloning vector for dominant negative mouse BMP receptor 1B

Depositor

Lee Niswander, Peter ten Dijke

Article

Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

Insert

BMPR-1B DN (Bmpr1b Mouse)

Expression

Bacterial

Mutation

dominant negative mutation (K231R); also L2V

Promoter

T3

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
GAL4-Creb5 (1- 508)

Plasmid

#195030

Purpose

GAL4-Creb5 fusion construct (full length, 508aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1_G2

Plasmid

#86610

Purpose

Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via nhej using ouabain

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

Plasmid

#199567

Purpose

Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer

Depositor

Andrea Cimarelli, Yuxin Song

Article

Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Trim69 isoform B (codon optimized) (TRIM69 Human)

Use

Retroviral; Allows for puromycin selection

Tags

Flag

Mutation

codon optimized

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-K660N

Plasmid

#45707

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660N

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-6p-hDcp2 E147/8Q

Plasmid

#72217

Purpose

bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

E147Q, E148Q and F404L (please see depositor'…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
B4GALT1-Dox

Plasmid

#124633

Purpose

Doxycycline-inducible B4GALT1 expression in mammalian cells

Depositor

Ron Weiss

Article

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Beta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635

Expression

Mammalian

Promoter

TRE-tight and hEF1a

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAMTS18 Wt

Plasmid

#29552

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wild type

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#108769

Purpose

Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa396

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Elafin

Plasmid

#18102

Depositor

Ronny Drapkin

Article

Labidi-Galy et al Oncogene. 2015 Jan 15;34(3):373-83. doi:

Insert

Elafin (PI3 Human)

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pRK5 Fyn SH2:R176E

Plasmid

#16035

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

SH2 point mutant: R176E

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v1 puro

Plasmid

#87792

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-eGFP.PB/PTK

Plasmid

#170540

Purpose

Expresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restored

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

Delta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS Jaws mCherry_tr

Plasmid

#111549

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBRPyCAG-fmKlf4-DsRed-Ip

Plasmid

#26274

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Klf4 (Klf4 Mouse)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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