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pT7-EGFP-C1-HsGIGYF2_AE

Plasmid

#148577

Purpose

Mammalian Expression of HsGIGYF2

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-GAP43-ECFP-stop-L2

Plasmid

#186356

Purpose

Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Growth-associated protein-43 (Gap43 Synthetic, Mouse)

Use

Expression of a fluorescent membrane marker

Tags

ECFP

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (692-839)

Plasmid

#188092

Purpose

To make monomeric ATG9 C-terminal tail for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-AC8(1-106)

Plasmid

#181958

Purpose

Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

EGFP-AC8(1-106) (Adcy8 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

Plasmid

#182560

Purpose

Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

Depositor

Zhe J. Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a ICL1
sfGFP-P2A-mCherry

Expression

Mammalian

Promoter

Syn

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-H58AF60AA64YV71Y-shRNA1res_W

Plasmid

#147902

Purpose

Mammalian Expression of HsNot9-H58AF60AA64YV71Y-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-H58AF60AA64YV71Y-shRNA1res (CNOT9 Human)

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-Y203AR205AH208AR244A-shRNA1res_W

Plasmid

#147907

Purpose

Mammalian Expression of HsNot9-Y203AR205AH208AR244A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-Y203AR205AH208AR244A-shRNA1res (CNOT9 Human)

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-R205AH208A-shRNA1res_W

Plasmid

#147903

Purpose

Mammalian Expression of HsNot9-R205AH208A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-R205AH208A-shRNA1res (CNOT9 Human)

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-Y203AR244A-shRNA1res_W

Plasmid

#147908

Purpose

Mammalian Expression of HsNot9-Y203AR244A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-Y203AR244A-shRNA1res (CNOT9 Human)

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_C

Plasmid

#146086

Purpose

Mammalian Expression of HsTNRC6A

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-FHA-Linker-eGFP

Plasmid

#176065

Purpose

EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

FHA

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET24a His6 GST PYL2(14-188)

Plasmid

#177842

Purpose

Bacterial Expression of PYL2

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 2 (PYL2 Mustard Weed)

Tags

6xHis-GST

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Grhl3

Plasmid

#172869

Purpose

Mammalian expression vector with myc-tagged mouse Grhl3 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Grhl3 (Grhl3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167848

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis and AviTag

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCDH_CMV_dimer_mKate2

Plasmid

#167333

Purpose

Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Changed K95A, Y99F, E106A, E112A and R116A

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_mKate2

Plasmid

#167334

Purpose

Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) and HMGA …

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMG058 MBP-GS 634-666-His

Plasmid

#154077

Purpose

Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GYS1 (GYS1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop

Plasmid

#153956

Purpose

Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 ORF7B C-trunc

Plasmid

#153957

Purpose

Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

June 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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