19,660 results

1. mCherry-Moesin-C-14

   Plasmid

   #55103

   Purpose

   Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Moesin (MSN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. psd44-GqQL

   Plasmid

   #46826

   Purpose

   2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   g protein alpha q (GNAQ Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Q209L (The mutation reduces GTPase activity resul…

   Promoter

   PGK promoter

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. T777T

   Plasmid

   #113082

   Purpose

   Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoV2-N-R203M

   Plasmid

   #177952

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203M) (N )
   

   Expression

   Mammalian

   Mutation

   R203M

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ptet-RFP-shR-rtTA

   Plasmid

   #35625

   Purpose

   Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

   Depositor

   Howard Gu

   Article

   Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

   Type

   Empty backbone

   Use

   AAV and RNAi; Tet inducible

   Expression

   Mammalian

   Promoter

   Ptet

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-CCO-IRES-Puromycin

   Plasmid

   #134861

   Purpose

   Expresses EGFP-tagged inner mitochondrial membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome c oxidase subunit VIII (COX8A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWZL Neo Myr Flag TBK1

   Plasmid

   #20648

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDV5 containing Sb-loop

   Plasmid

   #132695

   Purpose

   pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_loop including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-5FLAG-hphMX6

   Plasmid

   #19342

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   hphMX6
   

   Expression

   Yeast

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SHP2 WT

    Plasmid

    #8381

    Depositor

    Ben Neel

    Insert

    SHP2 (PTPN11 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTALYM3-Telo1

    Plasmid

    #47883

    Purpose

    TALE-mClover against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP3535

    Plasmid

    #46886

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Expression

    Bacterial

    Promoter

    PnisA

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6M-Pak1 K299R

    Plasmid

    #12210

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Catalytically inactive Pak1: K299R

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139090

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRetrosuper Myc shRNA

    Plasmid

    #15662

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    Myc shRNA (MYC Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166015

    Purpose

    mammalian expression of HCoV-OC43 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-OC43 S (spike)
    

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mRFP-UtrCH

    Plasmid

    #26739

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    monomeric RFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261)

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJ3M-Mst1 K59R

    Plasmid

    #12204

    Depositor

    Jonathan Chernoff

    Article

    Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

    Insert

    Mst1 (STK4, MST1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Catalytically inactive: K59R

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCBFD1/HBFD1Bad

    Plasmid

    #53364

    Purpose

    Will convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    cbfd1
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGal4BD-CRY2

    Plasmid

    #28243

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    Gal4BD

    Expression

    Yeast

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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