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Mito-ABKAR

Plasmid

#61509

Purpose

Expression of AMPK biosensor targeted to the mitochondria

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Mitochondrial targeting signal (DAKAP1-30)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pST1374-N-NLS-flag-linker-Cas9-H840A

Plasmid

#51129

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2

Plasmid

#16066

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Mutation

aa2-500, inserted alanin at aa434

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF9B

Plasmid

#141280

Purpose

Gateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF9B (N SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mGFP-10-sREACh-N3

Plasmid

#21947

Depositor

Ryohei Yasuda

Article

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

mGFP-10-sREACh

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-UTY-F

Plasmid

#17439

Purpose

Expression of UTY in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTY (UTY Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-EGFP

Plasmid

#53744

Purpose

Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

EGFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 27, 2014

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-hATG16A1

Plasmid

#58994

Purpose

Expresses SECFP-hATG16A1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)

Use

Retroviral

Tags

SECFP

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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1014 pcDNA3 T7 Akt1 K179M T308A S473A

Plasmid

#9031

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M T308A S473A (AKT1 Human)

Expression

Mammalian

Mutation

K179M T308A S473A

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Plk4(Sak) wt (Nigg HR9)

Plasmid

#41165

Depositor

Erich Nigg

Article

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 (PLK4 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKate2-CaURA3

Plasmid

#44878

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-human OCT4 PE-SV40-Luc

Plasmid

#52415

Purpose

Luciferase reporter plasmid for PE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Proximal element of hOCT4 promoter (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

Plasmid

#101218

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N692A, M694A, Q695A and H698A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

Plasmid

#40131

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

Depositor

Pawel Pelczar

Article

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Tags

FokI nuclease (KKR)

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pFNF

Plasmid

#22687

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Neo*

Use

Mouse Targeting

Expression

Bacterial and Mammalian

Mutation

FRT-pPGK-pEM7-Neo*-bpA-FRT

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV5 mouse src

Plasmid

#13663

Depositor

Joan Brugge, Peter Howley

Insert

src (Src Mouse)

Tags

Myr

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-2 myc

Plasmid

#11373

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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