We narrowed to 10,347 results for: plasmids 101

1. pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1

   Plasmid

   #190902

   Purpose

   AAV vector expressing thew GFP reporter gene and human sgHTT

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Insert

   GFP and sgHTT (HTT Human)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EFS and U6

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Coexpressed Free deadFPPS-NES

   Plasmid

   #182492

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   deadFPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHsSyx3-MBP

   Plasmid

   #179286

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   TEV protease cleavage site + mature maltose bindi…

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONOR-PARK6e5-I368N

   Plasmid

   #86154

   Purpose

   Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Insert

   PINK1 exon 5 homology arms (PINK1 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV2_hSyn_Aurora_CA_Citrine

   Plasmid

   #98219

   Purpose

   Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Aurora_C128A gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

   Promoter

   human synapsin

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR D-TOPO-C3_13

   Plasmid

   #60261

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   EDN3 enhancer (EDN3 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMasterE

   Plasmid

   #165080

   Purpose

   Episomal expression of reprogramming factors.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

   Inserts

   OCT4 (POU5F1 Human)
   SOX2 (SOX2 Human)
   KLF4 (KLF4 Human)
   MYC (MYC Human)
   LRH1 (NR5A2 Human)
   NANOG (NANOG Human)
   LIN28 (LIN28A Human)
   EGFP
   HSV TK
   

   Use

   Episomal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGL4.11-COL1A1WT

   Plasmid

   #230915

   Purpose

   Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

   Depositor

   Avi Rosenberg

   Article

   Yang et al Lab Invest. 2024 May;104(5):100336. doi:

   Insert

   Human COL1A1 promoter (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   luciferase - Luc2p

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   COL1A1

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSC-ISL1-T2A-LHX3

   Plasmid

   #90215

   Purpose

   To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

   Insert

   ISL1-T2A-LHX3 (ISL1, LHX3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSC-NGN2-IRES-GFP-T2A-Sox11

    Plasmid

    #90214

    Purpose

    To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

    Insert

    NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-tetO-NB

    Plasmid

    #197092

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223164

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC293 - pAAV EF1a V5-synuclein (WT)

    Plasmid

    #60057

    Purpose

    An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    alpha-synuclein (SNCA human, Human)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBK2045-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(CMV-gRNA1)

    Plasmid

    #223165

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting CMV

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid

    #111390

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    ChETA-mRuby2
    

    Use

    AAV

    Tags

    Myc and mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid

    #111391

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA-mRuby2
    

    Use

    AAV

    Tags

    Myc and mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL

    Plasmid

    #111392

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA-EYFP
    

    Use

    AAV

    Tags

    EYFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2-TetOminiCMV-SK

    Plasmid

    #136577

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-TetOminiCMV-KS

    Plasmid

    #136617

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Klf4-IRES-Sox2 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLPC-NMYC-hTRF1deltaBLM

    Plasmid

    #64165

    Purpose

    Retroviral vector expressing human TRF1 delta aa317-374 with N-terminal MYC tag

    Depositor

    Titia de Lange

    Article

    Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    deleted amino acids aa317-374 of human TRF1

    Promoter

    CMV

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only