3,636 results
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pHD-DsRed-attP-w+
Plasmid#80898PurposeVector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.DepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKC52
Plasmid#226780PurposeExpresses Drosophila UNC-104(R348G) in insect cellsDepositorInsertDrosophila unc-104 (R348G) (unc-104 Fly)
UseTagssfGFP and 2xStrepIIExpressionInsectMutationchanged Arginine 348 to GlycinePromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKC50
Plasmid#226778PurposeExpresses Drosophila UNC-104(V6M) in insect cellsDepositorInsertDrosophila unc-104 (V6M) (unc-104 Fly)
UseTagssfGFP and 2xStrepIIExpressionInsectMutationchanged Valine 6 to MethioninePromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Hy_pMT Laccase2 MCS Exon Vector
Plasmid#69884PurposeExpression plasmid for expressing circular RNAs of a desired sequence in DrosophilaDepositorInsertLaccase2 (stw Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pHD-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199203PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-intDepositorInsertT2A-split-intein-Gal4[1-20]-N-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[21-881]-C-int
Plasmid#199204PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-intDepositorInsertT2A-split-intein-Gal4[21-881]-C-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[1-20]-N-int
Plasmid#199201PurposeGateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expressionDepositorInsertsplit-intein-Gal4[1-20]-N-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
SGyA
Plasmid#160292PurposeDrives expression of Cas9 and a TdTomato fluorescent markerDepositorInsertsVas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome
UseTagsT2A GFPExpressionInsectMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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57F03_LexA-GAD
Plasmid#83256PurposeExpresses LexA-GAD under the control of 57F03 enhancerDepositorInsert57F03 enhancer
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3_NSnoFbox-vhhGFP4
Plasmid#35580DepositorInsertNSnoFbox-vhhGFP4 (slmb Fly)
UseTagsExpressionMammalianMutationF box domain of slmb deleted.PromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDmAct5C::Cas9-2A-Neo
Plasmid#176679PurposeExpression of Drosophila codon optimized spCas9 under Drosophila Act5C promoterDepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprTagsExpressionInsectMutationDrosophila codon optimizedPromoterD. melanogaster Act5CAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR
Plasmid#31222DepositorInsertCD4-tdTom (CD4 Fly, Human, Aequorea victoria)
UseTagsCD4 and tdTomatoExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …PromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUAS-syb:spGFP1–10
Plasmid#73698PurposeNeuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spGFP1–10 chimera
UseDrosophilaTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-V5
Plasmid#81172Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertHrp48-ADARcd fusion (Hrb27C Fly)
UseTagsExpressionInsectMutationPromoterDrosophila metallothionein promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-g-osk-TRICK
Plasmid#71244Purposeoskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.DepositorInsertoskar (osk Fly)
UseP element-mediated germline transformationTags12xPP7 and 6xMS2ExpressionInsectMutationPromoteroskAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only
