3,636 results

1. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFD3-ebony

   Plasmid

   #83380

   Purpose

   Drosophila gRNA expression plasmid targets ebony

   Depositor

   Richard Padgett

   Article

   Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

   Insert

   ebony (e Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5XUAS IVS CsChrimson tdtomato_tr

   Plasmid

   #111545

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChrimson_tdtomato_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SCP1-pUC119

   Plasmid

   #21846

   Depositor

   James Kadonaga

   Article

   Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

   Insert

   SCP1 (super core promoter 1)-pUC119
   

   Use

   Puc derivative - a cloning vector

   Available Since

   Feb. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD3-ovoD1

   Plasmid

   #111142

   Purpose

   Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

   Insert

   pCFD3-ovoD1 (ovo Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ovoD1 D1B2NR (18 kb)

   Plasmid

   #37280

   Depositor

   Norbert Perrimon

   Article

   Chou et al Development. 1993 Dec;119(4):1359-69.

   Insert

   ovoD1 genomic DNA fragment
   

   Expression

   Insect

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pKC31

   Plasmid

   #226777

   Purpose

   Expresses Drosophila UNC-104(R562H) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (R562H) (unc-104 Fly)
   

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Arginine 562 to Histidine (bris mutation)

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUASP dsRed Rab7 WT

   Plasmid

   #44824

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab7 (Rab7 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCFD3.1-w-dU6:3gRNA

   Plasmid

   #123366

   Purpose

   gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

   Depositor

   Simon Bullock

   Insert

   U6:3-gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Rab9

    Plasmid

    #31736

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab9 (Rab9 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

11. GFP-Rab5

    Plasmid

    #31733

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab5 (Rab5 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

12. K560GFP

    Plasmid

    #129761

    Purpose

    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHD-DsRed-attP-w+

    Plasmid

    #80898

    Purpose

    Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.

    Depositor

    Kate O'Connor-Giles

    Inserts

    attP
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    

    Use

    High copy, amp resistance

    Promoter

    3xP3 and GMR::hsp70

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p026 pCS2-EnR

    Plasmid

    #11028

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    engrailed (en Fly)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Mutation

    Engrailed repressor domain (bases encoding amino …

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-MXC

    Plasmid

    #37140

    Depositor

    Robert Duronio

    Article

    White et al J Cell Biol. 2011 May 16;193(4):677-94.

    Insert

    Multi Sex combs (mxc Fly)
    

    Use

    Gateway

    Available Since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. KHBD00188

    Plasmid

    #34340

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1034 (bcd Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASTattB-2xFlag-mCherry-Msp300KASH

    Plasmid

    #170807

    Purpose

    Expresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesis

    Depositor

    Vikki Weake

    Article

    Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

    Insert

    2xFLAG/mCherry-Msp300KASH
    

    Tags

    2xFLAG-mCherry and Msp300KASH

    Expression

    Insect

    Promoter

    5xUAS + hsp70 minimal promoter

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBP-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199201

    Purpose

    Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    split-intein-Gal4[1-20]-N-int
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBac-DsRed-ORCO_9kbProm-QF2

    Plasmid

    #104877

    Purpose

    Anopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoes

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    AgORCO-QF2
    

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid

    #163928

    Purpose

    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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