3,686 results
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Plasmid#63173Purposeubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassetteDepositorInsert"Flp-Out" GAL80
ExpressionInsectAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20X-UAS-ADARcd
Plasmid#81173Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertUAS-ADARcd
ExpressionInsectPromoterUASAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD7
Plasmid#73916PurposegRNA vector for AsCpf1DepositorInserttRNA:hAsCpf1-gRNA:tRNA
UseCRISPRExpressionInsectPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWG-dCas9-VPR
Plasmid#63802PurposeSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vectorDepositorInsertSP-dCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionInsectPromoteract5cAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dBrainbow
Plasmid#29327DepositorInsertUAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
ExpressionInsectAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
TagsN-myristoylationExpressionInsectAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ1208
Plasmid#44490DepositorInsertMinimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
UseGateway expression cloneAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-Brn2-DBD-EnR
Plasmid#19714DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationmouse Brn2 DNA binding domain-Drosophila engraile…Available sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDmelOR-mRho.V5.mER.hOr47a
Plasmid#126478PurposeHuman codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsH. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…ExpressionMammalianMutationcodon optimization for H. sapiens and codon optim…PromoterCMVAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-white[coffee]
Plasmid#84006PurposeDonor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4DepositorInsertA 2,080 bp fragment of the white[coffee] allele (w Fly)
UseUnspecifiedMutationA GC-to-AA mutation that creates a G589E missense…Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ1201
Plasmid#44483DepositorInsertMinimal Mos1, unc-119, peel-1, MCS
UseMultiple cloning site vectorAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAGO1
Plasmid#22768DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArgonaut-1, variant A (AGO1 Fly)
UseDrosophila expressionTagsEGFPAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
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K560GFP
Plasmid#129761PurposeExpresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assaysDepositorAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD4d-U6-1:white1-U6-3:white1
Plasmid#84005PurposepCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertswhite sgRNA-1
white sgRNA-1
UseCRISPRPromoterU6:1, U6:3, and dU6-1; dU6-3Available sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dmBACCS2-IRES-dOrai-IRES-GFP
Plasmid#72893PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdmBACCS2-IRES-dOrai-IRES-GFP
TagsIRES-GFPExpressionMammalianPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dmBACCS2-IRES-dOrai
Plasmid#67627PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdmBACCS2-IRES-dOrai
ExpressionMammalianPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
TagsN-myristoylationExpressionInsectAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by