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3,641 results

Showing: 61 - 80 of 3641 results

  2. K9L mutant of H3K9me3 biosensor

    Plasmid
    #120807
    Purpose
    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    Expression
    Mammalian
    Mutation
    lysine 9 is mutated to leucine 9 on histone H3
    Promoter
    CMV
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120807-plasmid-map-sequence-id-235134

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  3. pGEX-6P-1-DmPABPC1_J

    Plasmid
    #146647
    Purpose
    Bacterial Expression of DmPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    DmPABPC1 (pAbp Fly)
    Expression
    Bacterial
    Mutation
    G26S relative to NM_166265.2/NP_725750.1
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146647-plasmid-map-sequence-id-437529

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  5. W45A mutant of H3K9me3 biosensor

    Plasmid
    #120808
    Purpose
    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    Expression
    Mammalian
    Mutation
    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…
    Promoter
    CMV
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120808-plasmid-map-sequence-id-235136

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  6. pMesh1*

    Plasmid
    #175591
    Purpose
    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Tags
    Fused with a flexible GS linker to cerulean
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175591-plasmid-map-sequence-id-348365

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  15. pMT-IMD-V5-His

    Plasmid
    #63748
    Purpose
    Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    immune deficiency (imd Fly)
    Tags
    V5-6XHis
    Expression
    Insect
    Mutation
    *see comment below
    Promoter
    pMT
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63748-plasmid-map-sequence-id-108846

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  20. pUAS-kkv::NG

    Plasmid
    #138953
    Purpose
    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv (NEWENTRY Fly)
    Use
    Uas transgene vector
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Available Since
    Nov. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138953-plasmid-map-sequence-id-274788

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Showing: 61 - 80 of 3641 results