3,607 results

1. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

   Plasmid

   #63167

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-cMyc"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124434

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3_NSnoFbox-vhhGFP4

   Plasmid

   #35580

   Depositor

   Markus Affolter

   Article

   Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

   Insert

   NSnoFbox-vhhGFP4 (slmb Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F box domain of slmb deleted.

   Promoter

   Available since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-HemmarR

   Plasmid

   #31222

   Depositor

   Yuh-Nung Jan

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 May 23. (

   Insert

   CD4-tdTom (CD4 Aequorea victoria, Fly, Human)
   

   Use

   Tags

   CD4 and tdTomato

   Expression

   Insect

   Mutation

   Fusion of signal peptide of Drosophila Akh gene, …

   Promoter

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. NCD-GFP

   Plasmid

   #24507

   Depositor

   Ron Vale

   Article

   Case et al Cell. 1997 Sep 5. 90(5):959-66.

   Insert

   NCD (ncd Fly)
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pAC5.1-dCry-V5His

   Plasmid

   #136237

   Purpose

   Protein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tags

   Depositor

   Aziz Sancar, Steven Reppert

   Article

   Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

   Insert

   DmCry
   

   Use

   Tags

   His and V5

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM-11-6His-DmBelle_B

   Plasmid

   #145950

   Purpose

   Bacterial Expression of DmBelle

   Depositor

   Elisa Izaurralde

   Insert

   DmBelle (bel Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLucf

   Plasmid

   #37328

   Depositor

   John Schiller

   Article

   Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

   Inserts

   Luciferase (luciferase Fly)
   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SV40

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154787

   Purpose

   Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Synthetic, Fly)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and stdMCP

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

    Plasmid

    #63169

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-FLAG"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFD2-dU6:2gRNA

    Plasmid

    #49409

    Purpose

    expression of gRNA under control of the Drosophila U6:2 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-2:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-2

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid

    #163917

    Purpose

    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASp YFP Ras85D CA

    Plasmid

    #52247

    Purpose

    Drosophila expression of YFP-Ras85D constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. KHBD00188

    Plasmid

    #34340

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1034 (bcd Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. w+attB

    Plasmid

    #30326

    Depositor

    Jeff Sekelsky

    Insert

    phiC31 attB, hsp70::w
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT-Hrp48-ADARcd-V5

    Plasmid

    #81172

    Purpose

    identification of cell-specific targets of RNA binding proteins

    Depositor

    Michael Rosbash

    Article

    McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

    Insert

    Hrp48-ADARcd fusion (Hrb27C Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila metallothionein promoter

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUAST td tomato Rab5 WT

    Plasmid

    #58446

    Purpose

    expression of drosophila td tomato Rab5

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab5 (Rab5 Fly)
    

    Use

    Tags

    td tomato

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. nSYB-GAL80-DD

    Plasmid

    #124778

    Purpose

    Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GAL80 (GAL80-DD)
    

    Use

    Tags

    DD

    Expression

    Insect

    Mutation

    Promoter

    nSynaptobrevin

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid

    #126479

    Purpose

    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Inserts

    Or56a (Or56a Fly)
    Orco (Orco Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens and codon optim…

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUAST-yki:FLAG(3x)

    Plasmid

    #85622

    Purpose

    Express 3x Flagged yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Development. 2008 Mar;135(6):1081-8. doi

    Insert

    yki (yki Fly)
    

    Use

    Tags

    3x FLAG

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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