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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite

Plasmid

#248013

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly&activeSite

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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cASIC1 concatemer - fully assembled

Plasmid

#233133

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

Plasmid

#92400

Purpose

Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

Plasmid

#92401

Purpose

Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Promoter

bactin2

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

Plasmid

#185679

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

Plasmid

#134865

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Membrin (GOSR2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

Plasmid

#139015

Purpose

To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

HA-MAO

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

Plasmid

#185686

Purpose

Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163044

Purpose

CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

Plasmid

#185687

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EMB-COMP5AP-AviTag-9xHis

Plasmid

#157244

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EMB (EMB Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EMB-Fc(DAPA)-AviTag-6xHis

Plasmid

#156680

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EMB (EMB Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)

Plasmid

#160581

Purpose

Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminator

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

Luc

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (delta200)

Plasmid

#120278

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the delta200 Golgi-…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163043

Purpose

QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163063

Purpose

Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Gateway entry vector

Tags

eGFP

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pVT0494 MULTI-assembly vector with HuABC2 Variable Heavy Domain

Plasmid

#252076

Purpose

Vector for MULTI-assembly cloning for HuABC2 Variable Heavy Domain variants; replace HuABC2 insert with gene of interest via Gibson Assembly

Depositor

Patrick Hsu

Article

Tran et al Science. 2026 Feb 19:eaea1820. doi: 10.1

Insert

HuABC2 variable heavy domain

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

Plasmid

#158585

Purpose

Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

membrane localization signal-tagBFP-wrmScarlet11

Tags

tagBFP

Expression

Yeast

Mutation

GGGS linker

Promoter

TEF

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163062

Purpose

Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Gateway entry vector

Tags

mOrange2

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Supt6h-SH2

Plasmid

#46518

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Supt6h (SUPT6H Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1317-1441

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-hCRM1

Plasmid

#17647

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

CRM1 (XPO1 Human)

Tags

3x Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pET29b Tau Cys

Plasmid

#108867

Purpose

Expresses human Tau with a mutated cysteine

Depositor

Gabriele Kaminski Schierle

Insert

Tau (MAPT Human)

Expression

Bacterial

Mutation

I260C C291A C322A to allow maleimide dye labelling

Promoter

T7

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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His8-TEV-PseC

Plasmid

#89724

Purpose

Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseC (pseC Campylobacter jejuni)

Tags

His8 and TEV

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only
His8-TEV-PseH

Plasmid

#89725

Purpose

Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseH (pseH Campylobacter jejuni)

Tags

2xHis Gln 5xHis and TEV

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Pou6f1

Plasmid

#74356

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou6f1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

POU domain, class 6, transcription factor 1 (Pou6f1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET-Expotin 1

Plasmid

#42047

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Exportin-1 (XPO1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh3CDS

Plasmid

#192843

Purpose

Plasmid to produce ISH probe for mMyh3 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 3 (Myh3 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh3UTR

Plasmid

#192844

Purpose

Plasmid to produce ISH probe for mMyh3 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 3 (Myh3 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-XPO1-ts1

Plasmid

#174286

Purpose

XPO1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

XPO1 shRNA (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-XPO1-ts2

Plasmid

#174287

Purpose

XPO1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

XPO1 shRNA (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-XPO1-ts3

Plasmid

#174288

Purpose

XPO1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

XPO1 shRNA (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos3.2trLATm2allL+A

Plasmid

#203750

Purpose

Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLATm2(allL+A) (LAT Human)

Tags

mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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C1-CFP-Lyn11

Plasmid

#155234

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lyn11 membrane targeted CFP. Membrane control

Tags

CFP

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PCMV-intron myc Rab1aWT

Plasmid

#46776

Purpose

expression of WT Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (RAB1A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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We narrowed to 17,519 results for: Emb