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Showing: 121 - 160 of 253 results

  3. pJin-290

    Plasmid
    #125718
    Purpose
    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C
    Depositor
    Bryan Dickinson
    Article
    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437
    Insert
    PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125718-plasmid-map-sequence-id-244348

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  4. p5-71

    Plasmid
    #118085
    Purpose
    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    Depositor
    Bryan Dickinson
    Article
    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d
    Insert
    N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118085-plasmid-map-sequence-id-227374

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  6. p5-79

    Plasmid
    #118087
    Purpose
    N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid
    Depositor
    Bryan Dickinson
    Article
    Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d
    Insert
    N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118087-plasmid-map-sequence-id-229028

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  13. pNSSCPG

    Plasmid
    #183187
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183187-plasmid-map-sequence-id-366657

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  19. pLEW13

    Plasmid
    #24007
    Depositor
    George Cross
    Insert
    T7 RNA Polymerase
    Use
    Trypanosome expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2010
    Availability
    Academic Institutions and Nonprofits only
    24007-plasmid-map-sequence-id-11278

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  20. pETMCSI-T4P_Y320A

    Plasmid
    #122522
    Purpose
    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis
    Depositor
    Gottfried Otting
    Article
    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do
    Insert
    gp43 - DNA polymerase (43 Escherichia virus T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tyrosine 320 changed to alanine
    Promoter
    T7 promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122522-plasmid-map-sequence-id-238200

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  21. pNSSCP

    Plasmid
    #183188
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183188-plasmid-map-sequence-id-366663

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  29. pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

    Plasmid
    #231356
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231356-plasmid-map-sequence-id-459839

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  36. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  38. pET15bLCFPT7

    Plasmid
    #53545
    Purpose
    Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant
    Depositor
    Lingchong You
    Article
    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10
    Insert
    T7 RNAP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    53545-plasmid-map-sequence-id-83442

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Showing: 121 - 160 of 253 results