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Showing: 121 - 160 of 217 results

  2. pSECRETS-B

    Plasmid
    #196987
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    Spy gRNA cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196987-plasmid-map-sequence-id-389302

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  4. pJBL703

    Plasmid
    #140373
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-2BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140373-plasmid-map-sequence-id-297839

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  5. pEN41A-T38S38

    Plasmid
    #49524
    Purpose
    Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    constitutive TetR38
    Use
    Tags
    Expression
    Mutation
    Promoter
    Ptb38
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49524-plasmid-map-sequence-id-73916

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  6. pJBL704

    Plasmid
    #140374
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-4BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140374-plasmid-map-sequence-id-297844

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  7. pJBL705

    Plasmid
    #140375
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-5BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140375-plasmid-map-sequence-id-297846

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  8. pJBL710

    Plasmid
    #140380
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 14BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-14BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140380-plasmid-map-sequence-id-307671

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  9. pJBL702

    Plasmid
    #140372
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 0BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-0BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140372-plasmid-map-sequence-id-307666

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  10. pJBL706

    Plasmid
    #140376
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 6BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-6BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140376-plasmid-map-sequence-id-297859

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  11. pJBL707

    Plasmid
    #140377
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 8BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-8BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140377-plasmid-map-sequence-id-297840

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  12. pJBL708

    Plasmid
    #140378
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 10BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-10BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140378-plasmid-map-sequence-id-297838

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  13. pJBL709

    Plasmid
    #140379
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 12BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-12BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140379-plasmid-map-sequence-id-297860

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  15. pGMCK-T38S38-750-luxAB-DAS+4

    Plasmid
    #49518
    Purpose
    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    T38S8-P750-luxAB-DAS+4
    Use
    Tags
    DAS+4 (AANDENYSENYADAS)
    Expression
    Bacterial
    Mutation
    Promoter
    P750
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49518-plasmid-map-sequence-id-73896

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  16. pGMEH-T38M-750-luxAB-DAS+4

    Plasmid
    #49520
    Purpose
    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    T38M-P750-luxAB-DAS+4
    Use
    Tags
    DAS+4 (AANDENYSENYADAS)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49520-plasmid-map-sequence-id-73899

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  28. pGMCtN-TSC10M1-sspB

    Plasmid
    #49521
    Purpose
    Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    TSC10M1-sspB
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49521-plasmid-map-sequence-id-73901

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  29. pGMCtKq28-TSC10M1-sspB

    Plasmid
    #49526
    Purpose
    Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    TSC10M1-sspB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49526-plasmid-map-sequence-id-73918

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  30. pEN12A-P750

    Plasmid
    #49525
    Purpose
    Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    P750
    Use
    Tags
    Expression
    Mutation
    Promoter
    P750
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49525-plasmid-map-sequence-id-73917

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  34. pST_140_LVL2 cam

    Plasmid
    #179334
    Purpose
    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).
    Depositor
    Anke Becker
    Article
    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:
    Insert
    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…
    Promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179334-plasmid-map-sequence-id-357079

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  36. pcDNA4_ChR2opt_mKATE

    Plasmid
    #67958
    Purpose
    optical depolarization of mammalian cells
    Depositor
    Andreas Offenhäusser
    Insert
    Channelrhodopsin 2 opt
    Use
    Tags
    mKatushka
    Expression
    Mammalian
    Mutation
    synthetically produced for mammalian optimized co…
    Promoter
    CMV -TetON
    Available since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67958-plasmid-map-sequence-id-118415

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Showing: 121 - 160 of 217 results