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Showing: 151 - 200 of 12902 results

  5. pTSlb-wt

    Plasmid
    #60728
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60728-plasmid-map-sequence-id-99370

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  6. pZS1[FruR-L]

    Plasmid
    #60742
    Purpose
    Contains PIq driving expression of the FruR-L, the Fructose inducible chimera with the wt Lac DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Insert
    FruR-L
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    LacI/GalR repressor chimera. LacI DBD with FruR L…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60742-plasmid-map-sequence-id-97727

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  9. pzs1[GalS-L]

    Plasmid
    #60743
    Purpose
    Contains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Insert
    GalS-L
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    LacI/GalR repressor chimera. LacI DBD with GalS L…
    Promoter
    Available since
    July 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    60743-plasmid-map-sequence-id-97728

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  10. pZS1[RbsR-T]

    Plasmid
    #60751
    Purpose
    Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Insert
    RbsR-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60751-plasmid-map-sequence-id-97732

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  11. pZS1[RbsR-L]

    Plasmid
    #60741
    Purpose
    Contains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Insert
    RbsR-L
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    LacI/GalR repressor chimera. LacI DBD with RbsR L…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60741-plasmid-map-sequence-id-97726

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  12. S10A mutant of H3S10ph biosensor

    Plasmid
    #120810
    Purpose
    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Serine 10 is mutated to Alanine, and Threonine 3,…
    Promoter
    CMV
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120810-plasmid-map-sequence-id-235276

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  14. pTSlb-N748D

    Plasmid
    #60732
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60732-plasmid-map-sequence-id-99421

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  15. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60720-plasmid-map-sequence-id-97706

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  16. pTSlb-Q758C

    Plasmid
    #60729
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60729-plasmid-map-sequence-id-99392

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  17. pTSara-R756K

    Plasmid
    #60727
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60727-plasmid-map-sequence-id-97715

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  18. pTSlb-R756K

    Plasmid
    #60731
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60731-plasmid-map-sequence-id-99358

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  19. pTSara-N748D

    Plasmid
    #60726
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60726-plasmid-map-sequence-id-97714

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  20. pZS1[FruR-T]

    Plasmid
    #60752
    Purpose
    Contains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Inserts
    FruR-T
    FruR-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60752-plasmid-map-sequence-id-97733

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  24. pTSlb-R756S

    Plasmid
    #60730
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Jan. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60730-plasmid-map-sequence-id-97716

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  25. pTSara-R756S

    Plasmid
    #60725
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60725-plasmid-map-sequence-id-97713

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  26. pTSara-Q758C

    Plasmid
    #60724
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60724-plasmid-map-sequence-id-97710

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  27. Hygro-Cas9 donor

    Plasmid
    #86883
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
    Depositor
    Danwei Huangfu
    Article
    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86883-plasmid-map-sequence-id-173280

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  29. pZS1[TreR-T]

    Plasmid
    #60754
    Purpose
    Contains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Inserts
    TreR-T
    GalS-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60754-plasmid-map-sequence-id-97736

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  38. VHACg/pDONR221

    Plasmid
    #172094
    Purpose
    Genomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221
    Depositor
    George Haughn
    Article
    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.
    Insert
    VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    530bp upstream of VHA-C ATG
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172094-plasmid-map-sequence-id-345740

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  39. pZS1[GalS-T]

    Plasmid
    #60753
    Purpose
    Contains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Inserts
    GalS-T
    GalS-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    July 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    60753-plasmid-map-sequence-id-97735

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Showing: 151 - 200 of 12902 results