Addgene

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pCI-MEG3d

Plasmid

#59423

Purpose

Expresses lncRNA MEG3 isoform d in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3d (MEG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE

Plasmid

#233264

Purpose

Backbone plasmid used to clone lncRNA inserts in preparation for RMCE

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-NONHSAT073641

Plasmid

#92432

Purpose

mammalian expression of lncRNA NONHSAT073641

Depositor

Matthias Leisegang, Ralf Brandes

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

NONHSAT073641

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAGK112

Plasmid

#228127

Purpose

Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAGK111

Plasmid

#228126

Purpose

Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1

Plasmid

#86816

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAGK58

Plasmid

#228118

Purpose

Express node 814 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 814, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK65

Plasmid

#228123

Purpose

Express node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK59

Plasmid

#228119

Purpose

Express node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK57

Plasmid

#228117

Purpose

Express node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK56

Plasmid

#228116

Purpose

Express node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 412, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK61

Plasmid

#228121

Purpose

Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK60

Plasmid

#228120

Purpose

Express node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK62

Plasmid

#228122

Purpose

Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGK55

Plasmid

#228114

Purpose

Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

Plasmid

#86828

Purpose

Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSLS.419

Plasmid

#187208

Purpose

Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco4 ncRNA
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Direct-seq_lentiGuide-Puro-Loop2-8A8G

Plasmid

#157983

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Article

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (Loop2-8A8G)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Direct-seq_lentiGuide-Puro-Tail-8A8G

Plasmid

#157981

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Article

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (Tail-8A8G)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PB-Haunt E1-PuroT2AmCherry-4A KI

Plasmid

#92164

Purpose

For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt P-Inr2-cDNA KI homology arms

Use

Other

Tags

Expression

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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