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We narrowed to 2,478 results for: EFS

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Showing: 61 - 80 of 2478 results

  1. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

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  4. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92116-plasmid-map-sequence-id-177904

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  11. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222498
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    EFS
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222498-plasmid-map-sequence-id-441135

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  12. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222499
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    EFS
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222499-plasmid-map-sequence-id-441136

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  13. SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut

    Plasmid
    #169298
    Purpose
    Constitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    Arid3a-3'UTR-Mut (Arid3a Mouse)
    Use
    Lentiviral
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169298-plasmid-map-sequence-id-337864

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  14. SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut

    Plasmid
    #169296
    Purpose
    Constitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    ARID3A-3'UTR-Mut (ARID3A Human)
    Use
    Lentiviral
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169296-plasmid-map-sequence-id-337837

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  20. EFSp-GFP-YAP (5SA)

    Plasmid
    #174170
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    EFS
    Available Since
    Sept. 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174170-plasmid-map-sequence-id-343725

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Showing: 61 - 80 of 2478 results