We narrowed to 1,873 results for: FRT

1. Frt-hspB10

   Plasmid

   #63101

   Purpose

   Expression of HSPB10 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb10 (ODF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Frt-hspB6

   Plasmid

   #63097

   Purpose

   Expression of HSPB6 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb6 (HSPB6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Frt-hspB2

   Plasmid

   #63093

   Purpose

   Expression of HSPB2 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb2 (HSPB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Frt-hspB4

   Plasmid

   #63095

   Purpose

   Expression of HSPB4 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb4 (CRYAA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR{FRT}

   Plasmid

   #182163

   Purpose

   pENTR plasmid carrying yeast recombination site for Gateway cloning into introns of genes encoding fluorescent proteins

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   FRT
   

   Use

   Pentr plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. xCIAR_pcDNA5/FRT/TO

   Plasmid

   #86504

   Purpose

   Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   xCIAR (SOS1 Human)
   

   Use

   Frt

   Tags

   Expression

   Mammalian

   Mutation

   F929A and T968L in SOScat

   Promoter

   CMV (tet operator)

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

   Plasmid

   #110201

   Purpose

   Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   LexPR transactivator - 2A - Cerulean
   

   Use

   Tags

   Cerulean

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85008

   Purpose

   For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   EGFP-ires-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

   Plasmid

   #86230

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85038

    Purpose

    For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    FRT-stop-FRT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid

    #92140

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    AID[71-114]-eGFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

    Plasmid

    #156431

    Purpose

    Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    RAD21, Halotag (Rad21 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid

    #156452

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG

    Depositor

    Elphege Nora

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    RAD21, AID (Rad21 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

    Plasmid

    #156433

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    SORORIN, AID (Cdca5 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #86233

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

    Plasmid

    #175549

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    WAPL (Wapl Mouse)
    

    Use

    Mouse Targeting

    Tags

    AID[71-114]-eGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

    Plasmid

    #92143

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119067

    Purpose

    Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119065

    Purpose

    Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #232929

    Purpose

    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    OsTir1(F74G)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 74 to Glycine

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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