We narrowed to 24,970 results for: Mammalian expression plasmids

1. PBS/pU6- HIF1 alpha RNAi plasmid 1

   Plasmid

   #21103

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   RNAi against hypoxia inducible factor 1alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. PBS/pU6-HIF1 alpha RNAi plasmid 2

   Plasmid

   #21104

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   RNAi against hypoxia inducible factor 1alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. RNA motif plasmid EDEN15

   Plasmid

   #107252

   Purpose

   EDEN15 RNA motif

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   EDEN15 motif
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH-1362 scFv entry plasmid

   Plasmid

   #201912

   Purpose

   Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL3927 (pDonor(plasmid)_Pse)

   Plasmid

   #200905

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (pTarget NGS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV miRNA control plasmid

   Plasmid

   #31508

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

7. PAX6 CKO 2/7 Donor plasmid

   Plasmid

   #68467

   Purpose

   donor plasmid for targeting PAX6 locus

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   FRT flanked exon4 of PAX6 gene (PAX6 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK promoter

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

   Plasmid

   #170792

   Purpose

   This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BcLOV4 fusion tools screening plasmid #1

   Plasmid

   #174511

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. BcLOV4 fusion tools screening plasmid #2

    Plasmid

    #174512

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    

    Use

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. VE-cad-eGFP Donor plasmid

    Plasmid

    #92309

    Purpose

    Knock eGFP into human VE-cad locus

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad Homologous Recombination Arms (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    about 30 a.a. truncated at end of protein

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. RNA motif plasmid cloning backbone

    Plasmid

    #107253

    Purpose

    RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    RNA motif plasmid cloning backbone
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDY153: Flag(SUZ12) WT HDR plasmid

    Plasmid

    #170793

    Purpose

    WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. eIF3k sgRNA-2 PX459 plasmid

    Plasmid

    #192256

    Purpose

    expressing sgRNA-2 targeting eIF3k locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eIF3k sgRNA-1 PX459 plasmid

    Plasmid

    #192255

    Purpose

    expressing sgRNA-1 targeting eIF3k locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eIF3f sgRNA-1 PX459 plasmid

    Plasmid

    #192253

    Purpose

    expressing sgRNA-1 targeting eIF3f locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. eIF3f sgRNA-2 PX459 plasmid

    Plasmid

    #192254

    Purpose

    expressing sgRNA-2 targeting eIF3f locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. eIF3b sgRNA-2 PX459 plasmid

    Plasmid

    #192250

    Purpose

    expressing sgRNA-2 targeting eIF3b locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eIF3b sgRNA-1 PX459 plasmid

    Plasmid

    #192249

    Purpose

    expressing sgRNA-1 targeting eIF3b locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

    Plasmid

    #192239

    Purpose

    knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge