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pLV-mTurquoise2-Caspase-1-mNeonGreen
Plasmid#166784PurposeFRET analysis (pro caspase-1 cleavage) in living cellsDepositorInsertmTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
UseLentiviralTagsmNeonGreen and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-LRRK2 WD40
Plasmid#139211PurposeHuman LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.DepositorInsertLRRK2 (LRRK2 Human)
UseTagsN-His and MBPExpressionInsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-SARS-CoV-2-5'UTR
Plasmid#184638PurposeFluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTRDepositorInsertmScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPR-S12
Plasmid#84031PurposeTo episomally express codon optimized Cas9 and chimeric guide RNADepositorInsertshSpCas9
eGFP
UseTags3x FLAGExpressionMammalianMutationPromoterCMV and CMV (downstream of F2A self-cleaving pept…Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationfull-lengthPromotertac promoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
piB-GFP
Plasmid#13844DepositorInsertTATA-GFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOKD4
Plasmid#17214DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDB-His-MBP-mGSDMD
Plasmid#123365PurposeExpression of full-length mouse GSDMD in E. coliDepositorInsertGSDMD (Gsdmd Mouse)
UseTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S
Plasmid#166794PurposeMammalian cell expression vector for Flag-tagged DPP9SDepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOKD5
Plasmid#17215DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A
Plasmid#163988PurposeMammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tagDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationM1457APromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
UseTagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic-Mdm2-T3
Plasmid#32369DepositorInsertMdm2 promoter (Mdm2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic-Mdm2-T4
Plasmid#32370DepositorInsertMdm2 promoter (Mdm2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-C9ORF80
Plasmid#128418PurposeExpress C9ORF80 in E. coli with a His tag (C-terminal)DepositorInsertC9ORF80 (INIP Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
ASC-CASP1 Octamer
Plasmid#164032PurposeBacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamerDepositorUseTagsHis6-MBP-TEVExpressionBacterialMutationW169G (ASC), G20K (CASP1)PromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only
