We narrowed to 6,164 results for: cas9 expression plasmid

1. pEC1.2/EC1.1_spCas9_GFP

   Plasmid

   #161933

   Purpose

   CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC1.2/EC1.1_spCas9_TagRFP

   Plasmid

   #161934

   Purpose

   CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGW_RUBY_EggCas9

   Plasmid

   #225990

   Purpose

   To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas9-NAT

   Plasmid

   #64329

   Purpose

   Cas9 expression cassette carried by a yeast single-copy episome plasmid

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAS9i_TRP

   Plasmid

   #141253

   Purpose

   Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9id_TRP

   Plasmid

   #141254

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ACE2 g1 + Cas9

   Plasmid

   #153011

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ACE2 g4 + Cas9

   Plasmid

   #153014

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-dCas9-VP64-Hygro

   Plasmid

   #104405

   Purpose

   transient expression of dCas9-VP64 fusion protein

   Depositor

   Stephan Beck

   Insert

   VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnCas9-AID

    Plasmid

    #196242

    Purpose

    Plasmid expressing the nCas9-AID fusion

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    nickase Cas9 fused to AID
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG1guide_RUBY_EggCas9

    Plasmid

    #225983

    Purpose

    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants

    Depositor

    Imran Siddiqi

    Insert

    Guide RNA against AtAGAMOUS1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35s promoter to Drive RUBY and DD45p to drive Cas9

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-dCas9-SET7-Hygro

    Plasmid

    #104407

    Purpose

    transient expression of dCas9-SET7 fusion protein

    Depositor

    Stephan Beck

    Insert

    SET7 (SETD7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9_mouse_Adar1_ccB

    Plasmid

    #158122

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9_mouse_Adar1_ccA

    Plasmid

    #158120

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9_mouse_Adar1_ccC

    Plasmid

    #158121

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-Cas9-TagBFP

    Plasmid

    #236725

    Purpose

    Cloning vector Cas9-TagBFP

    Depositor

    David Bersten

    Insert

    Cas9-TagBFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-MP-84-spCas9-U6-gRNA

    Plasmid

    #206935

    Purpose

    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

    Depositor

    Markus Grompe

    Insert

    spCas9
    

    Use

    AAV

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    MP-84

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid

    #221503

    Purpose

    CRISPRi machinery fish codon optimized expressing plasmid

    Depositor

    Alessandro Ori

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    D10A and H839A point mutations

    Promoter

    Zebrafish Ubiquitin promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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