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pJL1 BSMT1

Plasmid

#106287

Purpose

Expresses BSMT1 in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

BSMT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCOLA kiwi sensor sfGFP

Plasmid

#107368

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

kiwi-detecting toehold + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pNP1 AND gate sfGFP

Plasmid

#107361

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

AND gate + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1 Aquamarine

Plasmid

#106285

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Aquamarine

Use

Tags

6xHis and TEV cleavage (N terminal on insert)

Expression

Bacterial

Mutation

T65S and H148G

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMiR-RECK-3'UTR-miR-21-mut

Plasmid

#53693

Purpose

Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding site

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Tags

Expression

Mutation

nucleotide sequence 1083-1086 is mutated to TTCG

Promoter

Available since

July 11, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-RECK-3'UTR-miR-7-mut

Plasmid

#53692

Purpose

Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-7 binding site

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Tags

Expression

Mutation

nucleotide position 171-174 are mutated to GAAG

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

Plasmid

#177360

Purpose

AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Inserts

tdTomato
Chimeric guide for SaCas9

Use

AAV

Tags

P2A and a tetracycline-controlled transactivator …

Expression

Mammalian

Mutation

Promoter

Synapsine promoter and U6 promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

Plasmid

#177365

Purpose

AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Inserts

tdTomato
Chimeric guide for SaCas9

Use

AAV

Tags

P2A and a tetracycline-controlled transactivator …

Expression

Mammalian

Mutation

Promoter

CMV and U6 promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pNP1 eforRed toehold switch

Plasmid

#107353

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 1 + eforRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 tdTomato toehold switch

Plasmid

#107354

Purpose

Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 5 + tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 Aqua toehold switch

Plasmid

#107356

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 8 + Aquamarine

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSMT3_SRSF1

Plasmid

#201056

Purpose

Express sumo-tagged human SRSF1

Depositor

Jun Zhang

Article

Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

Insert

SRSF1 (SRSF1 Human)

Use

Tags

sumo

Expression

Bacterial

Mutation

none

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-aadA

Plasmid

#117050

Purpose

In vitro expression of aadA (streptomycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

aadA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-neoR

Plasmid

#117048

Purpose

In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

neoR

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-SpCas9

Plasmid

#117051

Purpose

In vitro expression of S. pyogenes Cas9 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

S. pyogenes Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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U6-CjCas9-MS2gRNA-Backbone

Plasmid

#210711

Purpose

This plasmid express MS2 loop containing Cj Cas9 gRNA

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MS2 loop containing Cj Cas9 gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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EFS-SpdCas9

Plasmid

#210703

Purpose

This plasmid express EFS promoter driven human codon optimized SpdCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a core promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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