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pCI-neo-HA-hAtg13

Plasmid

#22874

Depositor

Noboru Mizushima

Article

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Insert

autophagy-related genes 13 (ATG13 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2010

Availability

Academic Institutions and Nonprofits only
pCI-Neo HA-hSki

Plasmid

#10910

Depositor

Bob Weinberg

Article

Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

Ski (SKI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-NHA-Ago2

Plasmid

#115361

Purpose

Expression vector to produce NHA-tagged human Ago2 protein

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

N peptide and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-HseIF4AI_AC

Plasmid

#148438

Purpose

Mammalian Expression of HseIF4AI

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HseIF4AI (EIF4A1 Human)

Use

Tags

Expression

Mammalian

Mutation

3 silent mutations relative to NM_001416.2

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-μ1A-(HA)3

Plasmid

#198177

Purpose

Expression of HA-tagged AP-1 μ1A in mammalian cells

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-HA-hApg12

Plasmid

#22950

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

autophagy related genes 12 (ATG12 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCI-Neo HA-hSnoN

Plasmid

#10908

Depositor

Bob Weinberg

Article

Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

SnoN (SKIL Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCI neo-hEST2-HA

Plasmid

#1782

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Clone is catalytically active but is dead in vivo.

Promoter

Available since

July 10, 2005

Availability

Academic Institutions and Nonprofits only
pCI.neo-rPAM1-M314I

Plasmid

#145802

Purpose

expresses catalytically inactive rat PAM-1 in mammalian cells

Depositor

Richard Mains, Betty Eipper

Article

Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Use

Tags

Expression

Mammalian

Mutation

changed methionine 314 to isoleucine to inactivat…

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsDCP1a_G

Plasmid

#146400

Purpose

Mammalian Expression of HsDcp1a

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and G1716T compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo.ARRDC1-mCherry

Plasmid

#38319

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-HA-hUVRAG

Plasmid

#24290

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV irradiation resistance-associated gene (UVRAG Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2010

Availability

Academic Institutions and Nonprofits only
pCI-neo-mApg5

Plasmid

#22956

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCIneoMyc mouse Numb

Plasmid

#41712

Depositor

Yutaka Hata

Article

Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

Insert

Numb (Numb Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 8-853

Promoter

CMV

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
pCIneo-NHA-LacZ

Plasmid

#115363

Purpose

Expression vector to produce NHA-tagged LacZ protein

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

lacZ beta-D-galactosidase

Use

Tags

N peptide and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-HseIF4AII_AC

Plasmid

#148439

Purpose

Mammalian Expression of HseIF4AII

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HseIF4AII (EIF4A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-AP-4 (Myc)3-σ4

Plasmid

#198331

Purpose

Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Use

Tags

triple Myc tag

Expression

Mammalian

Mutation

silent substitution in codon 131

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsSmg6_I

Plasmid

#146551

Purpose

Mammalian Expression of HsSmg6

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6 (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneoRL-FHL1 3'UTR

Plasmid

#84600

Purpose

Renilla luciferase reporter containing FHL1 3'UTR

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

FHL1 3'UTR (FHL1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mutation

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCIneoRL-BCL2 3'UTR

Plasmid

#84597

Purpose

Renilla luciferase reporter containing BCL2 3' UTR

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

BCL2 3'UTR (UTS2R Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mutation

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only