2,294 results

1. pCI-syn-fRGECO2

   Plasmid

   #125244

   Purpose

   Expresses red fluorescent calcium sensor in hippocampal slices

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRGECO2
   

   Expression

   Mammalian

   Mutation

   W44Y, D432A

   Promoter

   synapsin

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-IP-EGFP-LC3

   Plasmid

   #38195

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK283 (Stag-3FLAG-Neo)

   Plasmid

   #72799

   Purpose

   C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   Stag-3FLAG-Neo
   

   Use

   CRISPR

   Tags

   Stag-3FLAG

   Expression

   Mammalian

   Available Since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE GFP-Clta KI

   Plasmid

   #131483

   Purpose

   Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Clta Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRXIP Lamp1-Venus

   Plasmid

   #89937

   Purpose

   Expresses Lamp1 tagged with Venus in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

   Insert

   rat Lamp1 (Lamp1 Rat)
   

   Use

   Retroviral

   Tags

   Venus

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKC beta I CAT

   Plasmid

   #16380

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC beta 1 (Prkcb Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   CAT: catalytic domain, aa 329-671

   Available Since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pEF HaloTag-SYB2

   Plasmid

   #197282

   Purpose

   Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYB2 and HaloTag (Vamp2 Rat)
   

   Expression

   Mammalian

   Mutation

   Q76V and F77W

   Promoter

   EF1alpha

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Syt1 C2AB (96-421)

   Plasmid

   #170643

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. KIF5A-GFP-SspB

   Plasmid

   #214401

   Purpose

   Expresses fusion of Kinesin heavy chain 5A (1-572) with GFP and SspB (micro)

   Depositor

   Liting Duan

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

   Insert

   KIF5A-GFP-SspB (Kif5a Synthetic, Rat)
   

   Tags

   KIF5A-GFP-SspB

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-beta-arrestin1

    Plasmid

    #36918

    Depositor

    Robert Lefkowitz

    Article

    Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

    Insert

    beta-arrestin1 (Arrb1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pRSV-CaMKII

    Plasmid

    #45064

    Purpose

    Expression of CaMKII

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    CaM Kinase II (Camk2a Rat)
    

    Expression

    Mammalian

    Promoter

    RSV

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAMPK alpha2 delta312X

    Plasmid

    #60127

    Purpose

    expresses constitutively active AMPKalpha2

    Depositor

    Morris Birnbaum

    Insert

    AMPK alpha 2 (Prkaa2 Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Truncated at residue 312

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Kaede-Lysosomes-20

    Plasmid

    #57318

    Purpose

    Localization: Lysosome Membrane, Excitation: 508 / 572, Emission: 518 / 580

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Tags

    Kaede

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pORANGE Rims1-GFP KI

    Plasmid

    #131494

    Purpose

    Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rims1 Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK280 (mCherry2-NeoR)

    Plasmid

    #72796

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Bsr
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Wnk1

    Plasmid

    #38780

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

    Insert

    Wnk1 (Wnk1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCMV5-rat ERK2-WT

    Plasmid

    #40812

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

18. cacnb4 (rat) in pMT2 vector

    Plasmid

    #107426

    Purpose

    Expresses Calcium channel beta4 auxillary subunit in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

    Insert

    cacnb4 (Cacnb4 Rat)
    

    Expression

    Mammalian

    Promoter

    Ad MLP

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 HA-rnSOX9

    Plasmid

    #62972

    Purpose

    Expresses HA-tagged rat SOX9

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

    Plasmid

    #104493

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV Retrograde and AAV1

    Insert

    jGCaMP7b
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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