We narrowed to 1,322 results for: pET28a

1. pET-NEMO

   Plasmid

   #11966

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 13, 2006

   Availability

   Academic Institutions and Nonprofits only

2. Human USP30 (64-502, construct 13, MG-26-82)

   Plasmid

   #110746

   Purpose

   Bacterial expression of human USP30 (construct 13)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Use

   Tags

   N-His6-3C-GST-3C

   Expression

   Bacterial

   Mutation

   F348D, M350S, I353E + insertion deletion: 64-178;…

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-sfGFP-DogTag Loop B

   Plasmid

   #171780

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop B
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Asp1…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-GST-TNRC6A-(935-984)

   Plasmid

   #42050

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   contain aa935-984

   Promoter

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-sfGFP-SpyTag003 Loop A

   Plasmid

   #171776

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-SpyTag003 Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   SpyTag003 and linkers inserted between residues V…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLyGo-Ec-6

   Plasmid

   #163132

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HOXB13 (human) HIS-tag pET

   Plasmid

   #8543

   Depositor

   Corey Largman

   Article

   Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western and by transcription/tra…

   Promoter

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pET-NEMOL329P

   Plasmid

   #11969

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMOL329P (IKBKG Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Leucine329 to Proline

   Promoter

   Available Since

   July 13, 2006

   Availability

   Academic Institutions and Nonprofits only

9. Human USP30 (64-502, construct 13i, C77A, MG-31-28)

   Plasmid

   #110747

   Purpose

   Bacterial expression of human USP30 (construct 13i)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   C77A, F348D, M350S, I353E + insertion deletion: 6…

   Promoter

   T7

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-NFHΔ36-72

    Plasmid

    #232060

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain lacking residues 36-72
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-NFHΔ462-638

    Plasmid

    #232061

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain lacking residues 462-638
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-NFMtail

    Plasmid

    #232056

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    cDNA codon-optimized for E. coli; added N-termina…

    Promoter

    T7

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLyGo-Ec-4

    Plasmid

    #163130

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-sfGFP-DogTag Loop C

    Plasmid

    #171781

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Asp1…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-sfGFP-SpyTag003 Loop B

    Plasmid

    #171777

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues A…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-sfGFP-SpyTag003 Loop C

    Plasmid

    #171778

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues A…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLyGo-Ec-3

    Plasmid

    #163129

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLyGo-Ec-2

    Plasmid

    #163128

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLyGo-Ec-5

    Plasmid

    #163131

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK287

    Plasmid

    #71120

    Purpose

    Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCeSR beta deltaN

    Plasmid

    #13728

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (R186.3 Nematode)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    expresses residues 38-240 of C. elegans SRβ with …

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAB46 (CK2beta)

    Plasmid

    #27085

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CTB

    Plasmid

    #141166

    Purpose

    Expresses recombinant Cholera Toxin B Subunit

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    CTB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No secretion signal

    Promoter

    T7

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-AviTag-DogTag-MBP

    Plasmid

    #171773

    Purpose

    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogTag-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tUI

    Plasmid

    #122661

    Purpose

    Express tUI protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI (UBQLN1, USP5 Human)
    

    Use

    Tags

    His-tag

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pMIIS793

    Plasmid

    #238206

    Purpose

    Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Use

    Tags

    TwinStrepII-bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hCD12

    Plasmid

    #225541

    Purpose

    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Human HUWE1 (3993-4374)

    Plasmid

    #110754

    Purpose

    Bacterial Expression of HUWE1 HECT domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SpyTag003-OspC

    Plasmid

    #159998

    Purpose

    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag003-OspC (20-209) C129S
    

    Use

    Tags

    His6-tag and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    C129S mutation improves expression level.

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge