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Plasmid#91300PurposeProtein expression and purification of human SH3 domain construct FYB-2/2DepositorInsertFYB-2/2 (FYB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABI1-1/2
Plasmid#91306PurposeProtein expression and purification of human SH3 domain construct ABI1-1/2DepositorInsertABI1-1/2 (ABI1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)Double
Plasmid#100746PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorInsertAP2B1 (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoform with deletion of clathrin binding mo…PromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)Y815A
Plasmid#100744PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorInsertAP2B1 (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoform with Y815A mutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)∆CBM
Plasmid#100745PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorInsertbeta2 adaptin (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoform with deletion of clathrin binding mo…PromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl K
Plasmid#89380PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl F
Plasmid#89375PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl G
Plasmid#89376PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl J
Plasmid#89379PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl E
Plasmid#89374PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl I
Plasmid#89378PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl H
Plasmid#89377PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MLRV
Plasmid#118069PurposeMulti-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reportersDepositorInsertsTB:5xNF-κβ:RedF:bGHpA
TB:4xTGF-β:FLuc:bGHpA
TB:5xE-box:Renilla:bGHpA
TB:2xp53:NLuc:bGHpA
TB:6xAP-1:GrRenilla:bGHpA
CMV:ELuc:bGHpA
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoter2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL452-Sf3b1-K700E
Plasmid#90425Purposeselectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)DepositorInsertSf3b1 - left and right homology arms (Sf3b1 Mouse)
UseHomology-directed repair vectorTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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TFORF1706
Plasmid#143969PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertE2F1 (E2F1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE-8XGTIIC-DsRED-DD
Plasmid#115798Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporterDepositorInsertYAP responsive promoter (YAP1 Human)
UseLentiviralTagsDsRED-DHFRExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
BACE2_pcDNA6.2/EmGFP-Bsd
Plasmid#176942PurposeMammalian expression vector encoding BACE2 and EmGFP-BsdDepositorInsertBACE2 (BACE2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1-YAPS127A
Plasmid#166458PurposeExpresses fusion of mEGFP and YAPS127ADepositorInsertmEGFP, YAPS127A (YAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESF140_HisSpy_DDB1∆B
Plasmid#124213PurposeInsect cell expression vector for His6, Spy tagged hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTagsHis6-SpyExpressionInsectMutationinternal deletion of BPB domain to aid crystalliz…PromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1-YAP
Plasmid#166457PurposeExpresses fusion of mEGFP and YAPDepositorInsertmEGFP, YAP (YAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-YAP shRNA2
Plasmid#166485PurposeHuman YAP RNAiDepositorInsertshRNA for human YAP (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-YAP
Plasmid#166443PurposeBacterial expression of 6x His tagged YAPDepositorInsertYAP (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-neo-YAP
Plasmid#166468PurposePlasmid coding for YAP and neomycin resistance in a lentiviral backbone.DepositorInsertYAP, Neo (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-RNF2
Plasmid#31216DepositorInsertRNF2 (RNF2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-Yap shRNA
Plasmid#166486PurposeMouse Yap RNAiDepositorInsertshRNA for mouse Yap (Yap1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELK4
Plasmid#98617PurposeGateway entry TOPO TA cloning vector for ELK4DepositorInsertELK4 (Elk4 Mouse)
UseTopo ta cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)
Plasmid#139447PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-WT_RASA2-EF1-Puro
Plasmid#114096PurposeExpress RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)
Plasmid#139443PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX-0SB
Plasmid#169728PurposeMammalian expression construct of GFP tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-741 and has the major SB m…PromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g1 (BB34)
Plasmid#139452PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_ybbR_MAP7
Plasmid#159718PurposeExpresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tagDepositorInsertMAP7 (Microtubule Associated Protein 7) (MAP7 Human)
UseTagsZZ affinity tag, ybbR tagExpressionInsectMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416 ATG5-E141D-HA-ATG12-ATG16
Plasmid#166851PurposeExpresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.DepositorUseTagsHA-ATG12ExpressionYeastMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Double
Plasmid#100729PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motif and …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP ∆CBM
Plasmid#100728PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motifPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-S400F_RASA2-EF1-Puro
Plasmid#114098PurposeExpress mutant RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationS400FPromoterCMVAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Y215A
Plasmid#100727PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform Y215A mutation (Y815A in full length)PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234370PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantMutationPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234372PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-24XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantMutationPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5D
Plasmid#229415PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5D (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5A
Plasmid#229413PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5A (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3312
Plasmid#144788PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertELK4 (ELK4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T
Plasmid#191331PurposeExpresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
UseTagsExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644PromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0204
Plasmid#141554PurposeLentiviral vector for overexpressing the ELK4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertELK4 (ELK4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Shank2 scFv [N23B/6]
Plasmid#190543PurposeMammalian Expression of Shank2 scFV. Derived from hybridoma N23B/6.DepositorInsertShank2 (Rattus norvegicus) recombinant scFV (Shank2 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 20A
Plasmid#183639PurposeLentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-cDAXX-0SB
Plasmid#169730PurposeBacterial expression of N-terminally 6His tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTags6 HisExpressionBacterialMutationComprises residues 495-741 and has the major SB m…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-YAPL318E
Plasmid#166444PurposeBacterial expression of 6x His tagged YAPL318EDepositorInsertYAPL318E (YAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only