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pEF1α:flex-mcherry

Plasmid

#222618

Purpose

Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

Depositor

Franck Polleux

Article

Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

Insert

Cre-dependent mCherry

Use

AAV

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5 Fyn deltaSH3

Plasmid

#16034

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

Delta SH3: deletion of amino acids 76-141

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pRCPam

Plasmid

#195861

Purpose

Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.

Depositor

Gregory Phillips

Article

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Insert

Amber Chromogenic Protein on pRCPam plasmid

Expression

Bacterial

Mutation

Gln62X

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX C_K nsp1 CoV2

Plasmid

#175516

Purpose

Expresses C_K for bioconjugation on nsp1

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

C_K nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 Fyn SH2:R176E

Plasmid

#16035

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

SH2 point mutant: R176E

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pGEX nsp1 R99A CoV2

Plasmid

#175515

Purpose

For protein expression of R99A nsp1 CoV2

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

R99A nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX nsp1 K164A/H165A CoV2

Plasmid

#175514

Purpose

For protein expression of K164A/H165A nsp1 CoV2

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

K164A/H165A nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX nsp1 R124A/K125A CoV2

Plasmid

#175513

Purpose

For protein expression of R124A/K125A nsp1 CoV2

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

R124A/K125A nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX C_K nsp1 K164A/H165A CoV2

Plasmid

#175517

Purpose

Expresses C_K for bioconjugation on nsp1

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

C_K nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSN36

Plasmid

#100121

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to 1 VP16 domain (413-460) separated by a linker (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5-BCRn-CAGEn (Npu)

Plasmid

#241419

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product BCR-ABL, Figure 3, Extended Figure 6-7 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

BCRn-CAGEn (Npu) (BCR Synthetic, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo R99A nsp1

Plasmid

#175422

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R99A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSN34

Plasmid

#100119

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to 2 VP16 domains (413-460) separated by linkers (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00703

Plasmid

#39739

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3905 (Su(z)2 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00274

Plasmid

#34402

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1856 (ttk Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
PB-TAC-OK+9MS

Plasmid

#80482

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [1-483]

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pREPROII-attB

Plasmid

#86557

Purpose

Dox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxP

Depositor

Ralf Kuehn

Article

Merkl et al PLoS One. 2013;8(1):e55170. doi: 10.1371

Inserts

Oct4 (Pou5f1 Mouse)
cMyc (Myc Mouse)
Sox2 (Sox2 Mouse)
Kruppel-like factor 4 (Klf4 Mouse)

Use

Cre/Lox

Expression

Mammalian

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPAkt

Plasmid

#181914

Purpose

Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

InPAkt

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP Rootletin (Nigg pFL2(CW499))

Plasmid

#41166

Depositor

Erich Nigg

Article

Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

Insert

Rootletin (CROCC Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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GOLPH3 shRNA

Plasmid

#163860

Purpose

shRNA targeting the coding sequences of GOLPH3

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

golgi phosphoprotein 3 (GOLPH3 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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