We narrowed to 1,197 results for: Aspa

1. pDisplay FLIPE-10u

   Plasmid

   #13546

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207R in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-Tag2B Flag BimEL(TD)

   Plasmid

   #23092

   Depositor

   Roger Davis

   Article

   Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

   Insert

   Bim EL (TD) (Bcl2l11 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Threonine 112 to Aspartate

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDisplay FLIPE-100u

   Plasmid

   #13547

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   S118W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYes2-AtPCS1

   Plasmid

   #49766

   Purpose

   Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

   Depositor

   Julian Schroeder

   Article

   Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

   Insert

   AtPCS1 (PCS1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pQE80L SpyTag-ELP-SpyTag DK

   Plasmid

   #112635

   Purpose

   SpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DK

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   SpyTag-ELP-SpyTag DK
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Contains central cysteine. Reactive aspartic acid…

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-Dest53-SHP-1 S591D

   Plasmid

   #21803

   Depositor

   Stephen Shaw

   Article

   Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

   Insert

   SHP-1 (PTPN6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Serine 591 to Aspartic acid

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pDEST-Hemmar-FLP(D)

   Plasmid

   #119895

   Purpose

   FLP(D) with a hsp70 promoter

   Depositor

   Rita Sousa-Nunes

   Article

   Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

   Insert

   FLP(D)
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   mutated form of FLP, which at position 5 contains…

   Promoter

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSET FLIPE intramol-1m

   Plasmid

   #13551

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIP-E intramol A207W (gltI E. coli)
   

   Use

   Tags

   EYFP

   Expression

   Bacterial

   Mutation

   A207W, ECFP insert in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA6-rbN1 S77D

   Plasmid

   #32947

   Depositor

   Edward Weinman

   Article

   Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

   Insert

   slc9a3r1 (SLC9A3R1 rabbit)
   

   Use

   Tags

   T7 tag, his, and partial FLAG

   Expression

   Mammalian

   Mutation

   Serine 77 mutated to Aspartic Acid

   Promoter

   CMV

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC-EsaR-I70V/D91G

    Plasmid

    #47644

    Purpose

    pAC-EsaR with a208g and a272g mutations in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-I70V/D91G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Isoleucine 70 to Valine and Aspartic acid…

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pGSTag BimEL (T112D)

    Plasmid

    #23102

    Depositor

    Roger Davis

    Article

    Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

    Insert

    Bim EL (TD) (Bcl2l11 Mouse)
    

    Use

    Tags

    Gst

    Expression

    Bacterial

    Mutation

    Changed Threonine 112 to Aspartate

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pRSET FLIPE intramol-10u

    Plasmid

    #13550

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIP-E intramol A207R (gltI E. coli)
    

    Use

    Tags

    EYFP

    Expression

    Bacterial

    Mutation

    A207R, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TEG +H122D

    Plasmid

    #187434

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed histidine at 128 to aspartic acid

    Promoter

    PH and p10

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FER +H122D

    Plasmid

    #187428

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed histidine at 128 to aspartic acid

    Promoter

    PH and p10

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MON +N122H

    Plasmid

    #187433

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed asparagine at 128 to histidine

    Promoter

    PH and p10

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJCC_084 Cas12i1 D647A

    Plasmid

    #171671

    Purpose

    For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1 D647A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 647 to alanine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFB HTB_hMSH6_T1219D

    Plasmid

    #135466

    Purpose

    Expression human MSH6_T1219D mutant protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6_T1219D (MSH6 Human)
    

    Use

    Tags

    His-tag

    Expression

    Insect

    Mutation

    changed threonine to aspartic acid

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS-Dhh1-PA(D195A,E196A)(405)

    Plasmid

    #122065

    Purpose

    Expressing Dhh1-PA

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    Dhh1-PA (DHH1 Budding Yeast)
    

    Use

    Tags

    PA

    Expression

    Yeast

    Mutation

    Aspartic acid 195 to alanine; Glutamic acid 196 t…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK278

    Plasmid

    #71134

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to asparagine; additional N-…

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK591

    Plasmid

    #71589

    Purpose

    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes threonine-2 to alanine and aspartate-177 …

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge