We narrowed to 11,394 results for: SOM

1. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

   Plasmid

   #196879

   Purpose

   Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-rCM1 (Nedd1 Rat)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-Nedd1-mOrange2-rCM1

   Plasmid

   #196861

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-rCM1 (Nedd1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSF-MCM3/5

   Plasmid

   #116950

   Purpose

   Expresses human MCM3 and human MCM5

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM5 (MCM5 Human)
   human MCM3 (MCM3 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-MCM4/6

   Plasmid

   #116951

   Purpose

   Expresses human MCM4 and human MCM6

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM6 (MCM6 Human)
   Human MCM4 (MCM4 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET32-MCM2/7

   Plasmid

   #116949

   Purpose

   Expresses human MCM2 and human MCM7

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM7 (MCM7 Human)
   Human MCM2 (MCM2 Human)
   

   Use

   Tags

   Thioredoxin, His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCag FlpO-2A-Cre EV

   Plasmid

   #129419

   Purpose

   episomal expression of FlpO and Cre recombinases

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Lyso-INPP4B-mCherry

   Plasmid

   #184055

   Purpose

   Lysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   LGP120 Lyso-INPP4B-mCherry (LAMP1, INPP4B Human)
   

   Use

   Tags

   Lysosome-associated membrane protein 1 (LAMP1). a…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBactin-AcGFP-Cdk5rap2-CTD

   Plasmid

   #196853

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Adeno FT

   Plasmid

   #101824

   Purpose

   Adenovirus for the expression of gRNAs targeting intron 17 of murine Fgfr3 and intron 6 of murine Tacc3

   Depositor

   Andrea Ventura

   Article

   Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

   Insert

   U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 (Fgfr3, Tacc3 Mouse)
   

   Use

   Adenoviral

   Tags

   FLAG-Cas9

   Expression

   Mutation

   Promoter

   U6 and CBh

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pCag SE (Self-excising) FlpO-2A-Cre EV

    Plasmid

    #130986

    Purpose

    episomal expression of FlpO and Cre recombinases, self excising

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    FlpO-2A-Cre
    

    Use

    Cre/Lox and Unspecified; Episomal expression vect…

    Tags

    Expression

    Mammalian

    Mutation

    protamine intron added to FlpO to prevent bacteri…

    Promoter

    CMV/Chick β-actin (CAG)

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid

    #128386

    Purpose

    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption

    Depositor

    Craig Duvall

    Article

    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

    Inserts

    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Human, Synthetic)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    C-terminal luc2 fragment and N-terminal luc2 frag…

    Expression

    Mammalian

    Mutation

    N terminal carbohydrate recognition domain fused …

    Promoter

    EF1A and IRES

    Available since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUB39

    Plasmid

    #26300

    Depositor

    George Cross

    Insert

    VSG117 (Lister 427-4) cDNA
    

    Use

    Trypanosome inducible expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEW100v5-trunc

    Plasmid

    #33346

    Depositor

    George Cross

    Insert

    Luciferase truncated to allow cytoplasmic instead of peroxisomal (glycosomal) expression
    

    Use

    Trypanosoma brucei expression vector

    Tags

    Expression

    Mutation

    21bp of wt luciferase deleted

    Promoter

    Available since

    Dec. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS3ndd

    Plasmid

    #97354

    Purpose

    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant

    Depositor

    Jamorn Somana

    Insert

    Nucleoid Disruptive Domain
    

    Use

    Cloning

    Tags

    Expression

    Bacterial

    Mutation

    changed methionine 1 to isoleucine

    Promoter

    E. coli lacZ promoter

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS2ndd

    Plasmid

    #97353

    Purpose

    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, ampicillin resistant

    Depositor

    Jamorn Somana

    Insert

    Nucleoid Disruptive Domain
    

    Use

    Cloning

    Tags

    Expression

    Bacterial

    Mutation

    changed methionine 1 to isoleucine

    Promoter

    E. coli lacZ promoter

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK138

    Plasmid

    #73314

    Purpose

    Pas2p peroxisomal membrane targeting sequence

    Depositor

    T. J. Kappock

    Insert

    Pas2p (peroxisomal membrane targeting region)
    

    Use

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    encodes residues 1-42

    Promoter

    AOX1

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PP-ASL

    Plasmid

    #40749

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Trypanosoma Brucei Adenylosuccinate Lyase
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pJK139

    Plasmid

    #73315

    Purpose

    Pas2p peroxisomal membrane biogenesis protein

    Depositor

    T. J. Kappock

    Insert

    Pas2p peroxisomal membrane targeting protein
    

    Use

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    Encodes a glycine-204 to serine mutation

    Promoter

    AOX1

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PP-MetAP

    Plasmid

    #40745

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-MetAP
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pMasterE

    Plasmid

    #165080

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    LIN28 (LIN28A Human)
    EGFP
    HSV TK
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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