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pHTN-HaloTag_ACTB:1-375

Plasmid

#210970

Purpose

Mammalian expression of full-length human ACTB. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ACTB:M1-F375 (ACTB Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_DCAF1:1038-1400

Plasmid

#210963

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_CORO1A:1-461

Plasmid

#210964

Purpose

Mammalian expression of full-length human CORO1A. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CORO1A:1-461

Plasmid

#210965

Purpose

Mammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DCAF1:1038-1400

Plasmid

#210962

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DCAF15:1-600

Plasmid

#210958

Purpose

Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF15:M1-L600 (DCAF15 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_FBXW11:1-542

Plasmid

#210952

Purpose

Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW11:M1-R542 (FBXW11 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_SKIC8:1-305

Plasmid

#211038

Purpose

Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SKIC8:M1-I305 (SKIC8 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_COPB2:1-597

Plasmid

#211055

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

COPB2:M1-A597 (COPB2 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_RBBP4:1-425

Plasmid

#211037

Purpose

Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP4:M1-S425 (RBBP4 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSimpleII-NmCas9-FLAG

Plasmid

#47872

Purpose

FLAG tagged NmCas9 with no NLS.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW7:1-707

Plasmid

#211099

Purpose

Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_PLCG1:1-1291

Plasmid

#211089

Purpose

Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PLCG1:M1-L1291 (PLCG1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

isoform 2. I813T natural variant (VAR_011908)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_CCT5:1-541

Plasmid

#210978

Purpose

Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CCT5:M1-E541 (CCT5 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-N_FBXW7:1-707

Plasmid

#210946

Purpose

Mammalian expression of full-length human FBXW7. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBOH-LIC_DDB1:1-1140_delta:396-689

Plasmid

#210897

Purpose

Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)

Use

Tags

Expression

Insect

Mutation

loop deletion amino acids I396 to D689

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBiT3.1-C_FBXW7:1-707

Plasmid

#211104

Purpose

Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_FBXW7:1-707

Plasmid

#210951

Purpose

Mammalian expression of full-length human FBXW7. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBOH-MHL_EIF3B:127-814

Plasmid

#210909

Purpose

Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF3B:D127-E814 (EIF3B Human)

Use

Tags

MHHHHHHSSGRENLYFQGMQ

Expression

Insect

Mutation

Y760C sequence conflict

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFBOH-avi-TEV-LIC_DCAF12:1-453

Plasmid

#210940

Purpose

Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF12:M1-S453 (DCAF12 Human)

Use

Tags

Expression

Insect

Mutation

R131Q natural variant (VAR_035322)

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge