We narrowed to 2,981 results for: erf
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Plasmid#53559Purposebacterial expression of IFIT3DepositorAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only
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pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196084Purpose(Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseCRISPRExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300 (D1399Y)
Plasmid#191763PurposeExpression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)DepositorInsertFRB-EGFP-p300 core (D1399Y) (EP300 Human)
TagsEGFP (N-terminal of p300 core (D1399Y)) and FRB (…ExpressionBacterial and MammalianMutationcatalytic D1399Y mutation in p300PromoterEF1alphaAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
Available SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GDI2
Plasmid#67093PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_ELF2
Plasmid#187737PurposeMAC-tagged gene expression of human ELF2DepositorInsertELF2 (ELF2 Human)
ExpressionMammalianAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-neo
Plasmid#166467PurposeEmpty vector to express overexpression cDNADepositorTypeEmpty backboneUseLentiviralAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCORE-Kp53
Plasmid#72241PurposeContains the CORE cassette with KanMX4 and human p53 gene markersDepositorAvailable SinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2880
Plasmid#143652PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-3xSV40pA-L-td-sfGFP
Plasmid#133574PurposeCAG tdGFP cre/lox reporter piggyBac transposon (3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseCre/LoxExpressionMammalianAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EB3-mNeonGreen
Plasmid#191761PurposeExpression of mNeonGreen fused to EB3DepositorInsertEB3-mNeonGreen (MAPRE3 Human)
TagsmNeonGreenExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALPS vif
Plasmid#101328PurposeEncodes codon optimized NL4-3 vifDepositorInsertNL4-3 vif
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS vpr
Plasmid#101329PurposeEncodes codon optimized NL4-3 vprDepositorInsertNL4-3 vpr
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H
Plasmid#118229PurposeEmpty E. coli - B. burgdorferi shuttle vector; hygromycin B resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-PQRv3-RFP
Plasmid#73951PurposeProtein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.DepositorInsertssfGFP (superfolder GFP)
RFP
TagsA residual PQR peptide wii be left at the C-termi…ExpressionMammalianPromoterChicken beta actin and Chicken beta actin (shared…Available SinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroME2f-mRuby2-ST
Plasmid#170160PurposeCation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroME2f-mRuby2-ST
ExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-rnc-sfGFP
Plasmid#51560PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).DepositorInsertrnc (rnc E. coli)
TagsGLESTCRHASLAVLADERRFSA and superfolder GFPExpressionBacterialMutationContains additonal aa at the end of the sfGFP in …Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PACT
Plasmid#50352Purposebacterial expression of PACTDepositorAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAL-rfp_T
Plasmid#74075PurposepAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRDepositorInsertsgRNA rfp (T)
UseSynthetic BiologyExpressionBacterialPromoterptacAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA
Plasmid#108746PurposeExpresses aa271 to C-terminus of Cre recombinase fused to PYL CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only