159,481 results

1. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

   Plasmid

   #196415

   Purpose

   Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   Lck-smMyc
   

   Use

   AAV; Astrocyte-selective

   Tags

   Lck

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and GfaABC1D

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM-mTagBFP2

   Plasmid

   #228615

   Purpose

   Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_CMV_MeltNLS/NES-40-mCherry

   Plasmid

   #234066

   Purpose

   Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

   Depositor

   Lukasz Bugaj

   Article

   Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

   Insert

   MeltNLS/NES-40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Beta-2-adrenergic receptor-mRFP

   Plasmid

   #67145

   Purpose

   Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Beta-2-adrenergic receptor-mRFP (ADRB2 Discosoma sp., Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-KRAB-MeCP2

   Plasmid

   #110821

   Purpose

   Improved dCas9 repressor-dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

   Plasmid

   #134860

   Purpose

   Expresses EGFP-tagged recycling endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAB11B (RAB11B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/AUG-DAP5-3XFLAG

   Plasmid

   #127331

   Purpose

   Express 3xFLAG tagged DAP5 protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   DAP5 (EIF4G2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FCHo2-pmCherryC1

   Plasmid

   #27686

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   FCHo2 (Fcho2 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-FR-GECO1a

    Plasmid

    #163681

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1a

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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