We narrowed to 8,596 results for: Set

1. pMyc-CMV1-huTLR4mut-C2141A

   Plasmid

   #53527

   Purpose

   expression of mutant TLR4 in mammalian cells

   Depositor

   Linda Yu

   Article

   Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

   Insert

   TLR4 C2141A (TLR4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C2141A

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3-HPRT1-S104Rf-PdTK-mCh

   Plasmid

   #107277

   Purpose

   HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Inserts

   HPRT genomic DNA (HPRT1 Human)
   puro-deltaTK; CAG::mCherry
   

   Mutation

   c.306G>T, c.312C>A

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRT1-Mb2YFP-2A (IG153)

   Plasmid

   #99619

   Purpose

   To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2EYFP
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRT2-Mb2Tomato-2A (IG154)

   Plasmid

   #99620

   Purpose

   To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2Tomato
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRT3-HA-H2B-Cerulean-2A (IG158))

   Plasmid

   #99624

   Purpose

   To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   HA-H2B-Cerulean
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMyc-CMV1-huTLR4mut-C1196T

   Plasmid

   #53526

   Purpose

   expression of mutant TLR4 in mammalian cells

   Depositor

   Linda Yu

   Article

   Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

   Insert

   TLR4 C1196T (TLR4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C1196T

   Promoter

   CMV

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMyc-CMV1-huTLR4mut-A896G

   Plasmid

   #53525

   Purpose

   expression of mutant TLR4 in mammalian cells

   Depositor

   Linda Yu

   Article

   Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

   Insert

   TLR4 A896G (TLR4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A896G

   Promoter

   CMV

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEf1a-Dn29-dCas9-P2A-GFP

   Plasmid

   #247155

   Purpose

   Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   Dn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKMW122_SpG-Cas9_U6-entry

   Plasmid

   #244819

   Purpose

   Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpG Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PC1575

    Plasmid

    #232088

    Purpose

    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

    Depositor

    Pranam Chatterjee

    Article

    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

    Insert

    uAb with cloning Esp3I restriction site
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-AtmiR173aTS-B/c

    Plasmid

    #227964

    Purpose

    Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDC32B-AtmiR173aTS-B/c

    Plasmid

    #227965

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Expression

    Plant

    Promoter

    35S and None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-NbmiR482aTS-B/c

    Plasmid

    #227966

    Purpose

    Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. MKC175_HBA1reg(synEPOR)

    Plasmid

    #232413

    Purpose

    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each.

    Depositor

    Kyle Cromer

    Article

    Shah et al bioRxiv 2024.04.12.589260

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid

    #232931

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Flag-Halo-CTCF ZF1 deletion mutant
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    Deleted amino acids 264-277

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

    Plasmid

    #233038

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and CasRx
    

    Use

    AAV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdnCas3-APOBEC1

    Plasmid

    #229537

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv2_sgALDH7A1

    Plasmid

    #218273

    Purpose

    CRISPR KO plasmid for ALDH7A1 in human cell lines

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    Inserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRLSIN-[EF1α-CD19 4-1BB]

    Plasmid

    #218340

    Purpose

    Lentiviral transfer plasmids for mammalian expression CD19 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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