We narrowed to 9,980 results for: Uty

1. pMRE146

   Plasmid

   #118498

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Expression

   Bacterial

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. c3GIC9n

   Plasmid

   #62192

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9 (D10A)
   

   Use

   CRISPR

   Tags

   HA

   Mutation

   D10A substitution (Cas9n)

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLX307 P2A GFP PRDM14

   Plasmid

   #193676

   Purpose

   Constitutive lentiviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hCMV-IE1:GLuc:bgH

   Plasmid

   #135294

   Purpose

   Transcriptional unit for constitutive expression of GLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:GLuc:bGH
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUB-sSypHer (RbcS-SypHer)

   Plasmid

   #84732

   Purpose

   Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   sSypHer
   

   Tags

   RbcS targeting sequence

   Expression

   Plant

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHelper-CBE-sgRNA

   Plasmid

   #221137

   Purpose

   Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with Cas9 handle
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI)

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1V5-His Snk/Plk2 T239D

   Plasmid

   #22220

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, threonine …

   Available Since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCR3.1 GSK3beta S9A HA

   Plasmid

   #15994

   Depositor

   Morris Birnbaum

   Article

   Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

   Insert

   GSK3beta (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S9A: constitutively active

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pET151-D-TOPO-PARP14-SΔK

   Plasmid

   #216658

   Purpose

   Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

   Depositor

   Herwig Schüler

   Article

   Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

   Insert

   PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   Deletion starting from Q1390 to D1448 and substit…

   Promoter

   T7

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHES941

    Plasmid

    #87942

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)
    

    Expression

    Yeast

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBabe HcRed Ras

    Plasmid

    #10678

    Depositor

    William Hahn

    Article

    Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

    Insert

    Hras V12 (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V, constitutively active

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

12. RIPR-bio-His

    Plasmid

    #50824

    Purpose

    Expresses enzymatically monobiotinylated full length RIPR

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    RIPR
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140222

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid

    #102895

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPH-T2A-GFP-shP53
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRE163

    Plasmid

    #118511

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Expression

    Bacterial

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMRE160

    Plasmid

    #118508

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Expression

    Bacterial

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSW002-PpsbA-E2-Crimson

    Plasmid

    #111258

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with E2-Crimson

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    E2-Crimson
    

    Expression

    Bacterial

    Promoter

    PpsbA

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMRE153

    Plasmid

    #118503

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Expression

    Bacterial

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPMVAd36

    Plasmid

    #98302

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

    Expression

    Yeast

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPotef-cdc3CB16-NatR

    Plasmid

    #86465

    Purpose

    Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

    Depositor

    Michael Feldbrügge

    Article

    Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

    Insert

    Cdc3
    

    Use

    Ustilago maydis expression

    Tags

    mCherry

    Expression

    Bacterial

    Promoter

    Potef

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge