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pDONR223_MAX_p.R35L

Plasmid

#82732

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway entry vector

Mutation

R35L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.W450L

Plasmid

#82830

Purpose

Gateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

W450L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLCKO-CMV-cMyc-UBC_K7R-IRES-Blasti

Plasmid

#242463

Purpose

Expresses human UBC protein with an N-terminal cMyc tag enabling UBC pull down along with a blasticidin selection marker. The K7R mutations prevent ubiquitin chain elongation.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

UBC (UBC Human)

Use

Lentiviral

Tags

cMyc

Expression

Mammalian

Mutation

UBC-K7R

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-Sun1-2xsfGFP-6xMYC-pA (JDW 694)

Plasmid

#242588

Purpose

A CMV driven expression vector containing a Sun1-2xsfGFP for labeling nuclear envenlope.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Sun1-2xsfGFP-6xMYC (Sun1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(YYFF)-mRuby2

Plasmid

#215447

Purpose

Gateway entry vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Tags

mRuby2

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

Plasmid

#229816

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

Plasmid

#229841

Purpose

A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-H2B-mCerulean-2xStop-loxP

Use

Cre/Lox; Tol2 based expression vector

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRev-erba-hSyn-mCherry-KASH

Plasmid

#223226

Purpose

guideRNA targeting the mouse Rev-erb alpha (Nr1d1)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Nr1d1 (Nr1d1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-rCD20-BSD

Plasmid

#209752

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXI3

Plasmid

#101518

Purpose

Donor Vector containing FOXI3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXI3 (FOXI3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ASCL3

Plasmid

#101515

Purpose

Donor Vector containing ASCL3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ASCL3 (ASCL3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXB3

Plasmid

#101478

Purpose

Donor Vector containing HOXB3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXB3 (HOXB3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXE1

Plasmid

#101439

Purpose

Donor Vector containing FOXE1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXE1 (FOXE1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXE3

Plasmid

#101429

Purpose

Donor Vector containing FOXE3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXE3 (FOXE3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXH1

Plasmid

#101431

Purpose

Donor Vector containing FOXH1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXH1 (FOXH1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-BSX

Plasmid

#101410

Purpose

Donor Vector containing BSX transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

BSX (BSX Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXB9

Plasmid

#101398

Purpose

Donor Vector containing HOXB9 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXB9 (HOXB9 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXC5

Plasmid

#101399

Purpose

Donor Vector containing HOXC5 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXC5 (HOXC5 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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