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pCAG-Ace2_D92N_E199V-mNeon

Plasmid

#129256

Purpose

Express Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Para

Plasmid

#218363

Purpose

Empty backbone for Tn7-site insertion with araC-ParaBAD expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Vinculin tail

Plasmid

#107154

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Tags

GST

Expression

Bacterial

Promoter

Ptac

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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XLone-BSD SARS-CoV2 N P2A mCherry

Plasmid

#154398

Purpose

PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry

Depositor

Xiaoping Bao

Insert

SARS-CoV2 N Protein (N SARS-CoV2)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE-Bi-SG-T

Plasmid

#26084

Depositor

Liqun Luo

Article

Li et al PLoS One. 2010 . 5(7):e11503.

Insert

Synaptophysin (Syp Mouse)

Tags

mut4EGFP

Expression

Mammalian

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PSD95-GCaMP2

Plasmid

#18931

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

PSD95-GCaMP2 (Dlg4 Rat)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pJOG130

Plasmid

#105336

Purpose

Gateway entry clone generation

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAG1244 p65-mCherry-FIREtag

Plasmid

#205175

Purpose

Expresses p65-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

p65-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pVW293

Plasmid

#139490

Purpose

Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pHSP12 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pHSP12

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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AiRT_RTctrl

Plasmid

#214070

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-DmrA

Plasmid

#61680

Purpose

GFP-labeled SAH scaffold with DmrA attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-DmrA

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62 L343A

Plasmid

#38279

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed L343A

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-Dppa3

Plasmid

#13365

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 3 (Dppa3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
LZF40: hSyn-Cre

Plasmid

#104124

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

Cre

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTK295

Plasmid

#59564

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pDM1038

Plasmid

#108988

Purpose

Inducible expression vector (dox-on)

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pERB215

Plasmid

#61499

Purpose

Const. Halotag-GFP-Nuf2 + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-Nuf2
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

Plasmid

#84248

Purpose

Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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