We narrowed to 10,491 results for: fus
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Plasmid#198048PurposeMoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyPromoterZea mays (maize) ubiquitin promoterAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL1P5 ZmUbiP:GRF-GIF:NosT
Plasmid#198046PurposeMoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyPromoterZea mays (maize) ubiquitin promoterAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDTFendoNA2
Plasmid#174731PurposeExpresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.DepositorInsertDT386F
ExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAF197
Plasmid#135930PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).DepositorInsertCTLA4 fused to IgG Fc and to mCherry
UseTransposonTagsmCherryExpressionMammalianPromoterEF1aAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
hRad51(A190L-A192L)–Cas9(D10A)
Plasmid#125562PurposeExpresses the Rad51(A190L-A192L)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-ERK:MEK
Plasmid#114291PurposeTET-inducible expression of ERK:MEK fusion proteinDepositorInsertrERK2 and hMEK1 proteins (Mapk1 Rat, Human)
UseLentiviralMutationrat ERK2 fused to human MEK1 with a linker in bet…Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA1-BE3
Plasmid#113418PurposeExpresses hA1-BE3 in mammalian cellsDepositorInserthA1-BE3 (APOBEC1 S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSN33
Plasmid#100118Purposeexpresses a chimeric KLF4-repressor fusionDepositorUseLentiviralExpressionMammalianAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268
Plasmid#69224PurposeExpresses R1335K mutant SpCas9 fused to Zif268 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLS and Zif268ExpressionMammalianMutationArginine 1335 is changed to LysineAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx30
Plasmid#69022PurposeExpresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49228PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCUPI NPD-GFP
Plasmid#1191DepositorInsertN-terminus of Sup35 (SUP35 Budding Yeast)
UseCup1 promoterTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mScarlet-CAAX2 WPRE
Plasmid#236232PurposeAAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2DepositorInsertmScarletI fused to the plasma membrane targeting sequence CAAX2
UseAAVTagsmScarletIPromoterhuman Synapsin 1Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pStreptagIIx2 Alfatag 3C InsR EABR
Plasmid#234996PurposeFor production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surfaceDepositorAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hPGRN
Plasmid#213682PurposeExpresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tagDepositorInsertHuman Progranulin (hPGRN) (GRN Human)
UseAAVTagsTwin-Strep tag and V5 tag (after signal peptide)Promotercytomegalovirus enhancer/chicken β-actin promoterAvailable SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
TagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF139
Plasmid#135924PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mTagBFP
UseTransposonTagsmTagBFPExpressionMammalianPromoterEF1aAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.mIKKα
Plasmid#74413PurposeExpresses mouse eGFP-IKKα in mammalian cellsDepositorAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY-tTA (B702)
Plasmid#92032PurposeExpresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activatorDepositorInsertCRY2 (CRY2 Mustard Weed)
TagstTA2 (TetR fused to VP64)ExpressionMammalianMutationNLS on CRY2 is mutatedPromoterCMVAvailable SinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-V5-6xHis
Plasmid#48346PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway CloningMutationContains a Kozak sequence and initiation codon up…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only