We narrowed to 11,714 results for: NSI

1. optoFcR

   Plasmid

   #224291

   Purpose

   Expression of an optogenetic Fc Receptor

   Depositor

   Meghan Morrissey

   Article

   Bond et al Dev Cell. 2024 Aug 9:S1534-5807(24)00457

   Insert

   Fc receptor gamma (Fcer1g Mouse)
   

   Use

   Lentiviral

   Tags

   mScarlet-Cry2-olig and myristoylation sequence

   Mutation

   aa 45-86 only

   Promoter

   SFFV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-ZNF251

   Plasmid

   #101451

   Purpose

   Donor Vector containing ZNF251 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF251 (ZNF251 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GRAB_CRFmut

   Plasmid

   #208659

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-TetO-hKLF4

   Plasmid

   #206386

   Purpose

   Expresses human KLF4 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223 SARS-CoV2 TEV-ORF9B

   Plasmid

   #154425

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9B
   

   Use

   Gateway-compatible entry vector, with insert of o…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   July 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

   Plasmid

   #75122

   Purpose

   Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   3x FLAG-tag

   Available Since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP Cep170 C-term (Nigg GG2)

   Plasmid

   #41151

   Depositor

   Erich Nigg

   Article

   Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

   Insert

   Cep170 (CEP170 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains C-terminal fragment (aa 755-1460, from X…

   Promoter

   CMV, T7

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

8. MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

   Plasmid

   #75115

   Purpose

   Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Mutation

   deleted bromodomain

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLV-TetOn-ND-Cdt1-mCherry

   Plasmid

   #193761

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1-mCherry (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3xFLAG CMV14 / BRAF-D594A

    Plasmid

    #131717

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D594A

    Promoter

    CMV

    Available Since

    Nov. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBacI-KDAC8 H143A

    Plasmid

    #224344

    Purpose

    Expresses human KDAC8 (HDAC8) H143A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC8 (HDAC8 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 143 mutated to alanine

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR207 SARS-CoV-2 NSP3 Cys857Ala

    Plasmid

    #154027

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP3
    

    Use

    Gateway-compatible entry vector, with mutagenized…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA8-Flag-LARS(1-976aa)

    Plasmid

    #139689

    Purpose

    Expresses N-teminal Flag tagged aa 1-976 of LARS1

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    LARS1 (LARS1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223 SARS-CoV-2 N_nostop

    Plasmid

    #152987

    Purpose

    Gateway-compatible Entry vector, with insert of N gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    N
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDONR223-ZNF706

    Plasmid

    #88037

    Purpose

    Donor Vector containing ZNF706 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF706 (ZNF706 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTWIN1-His6-Ssp-Httex1-7Q

    Plasmid

    #84347

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal His6-tagged intein Ssp

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3xFLAG CMV14 / BRAF-G469A

    Plasmid

    #131711

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    G469A

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid

    #159364

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3xFLAG CMV14 / BRAF-D594N

    Plasmid

    #131713

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D594N

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EFS-DIO-GRAB_CRFmut

    Plasmid

    #208663

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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