We narrowed to 11,714 results for: NSI

1. pAAV-Ef1a-Coff/Fon-oScarlet

   Plasmid

   #137138

   Purpose

   Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-oScarlet
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   E95D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-Myc-PGK-Puro-IRES-GFP

   Plasmid

   #75124

   Purpose

   Retroviral expression plasmid encoding wild type murine Myc

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Tags

   None

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

   Plasmid

   #154029

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP3
   

   Use

   Gateway-compatible entry vector, with mutagenized…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. TVBB C-term-mTurquoise2-Puro

   Plasmid

   #169224

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-ZNF692

   Plasmid

   #88701

   Purpose

   Donor Vector containing ZNF692 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF692 (ZNF692 Human)
   

   Use

   Gateway donor vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-Med25-VBD-6His

   Plasmid

   #64773

   Purpose

   Bacterial expression of His-tagged Med25-VBD

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25-VBD (MED25 Human)
   

   Expression

   Bacterial

   Mutation

   insert is 394-543 only

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. VBS mutant of alpha catenin sensor

   Plasmid

   #71710

   Purpose

   This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   alpha-catenin ΔVBS FRET Sensor
   

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Deleted amino acid residues 316-405 in α -catenin…

   Promoter

   CMV

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL2-Mcl-1 mut

   Plasmid

   #19133

   Depositor

   Wafik El-Deiry

   Article

   Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

   Insert

   Mcl-1 promoter (MCL1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   NFkB binding-site mutant, the sequence GGGGTCTTCC…

   Available Since

   Oct. 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-HOXD13-IDR-mCherry-CRY2-NLS

   Plasmid

   #145260

   Purpose

   Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13wt-IDR (HOXA13 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_BRD003 sgKEAP1-1

    Plasmid

    #74985

    Purpose

    sgKEAP1-1, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW-TetO-Gata3

    Plasmid

    #128824

    Purpose

    dox-dependent expression of mouse Gata3

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Lentiviral

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-TetO-Eomes

    Plasmid

    #128825

    Purpose

    dox-dependent expression of mouse Eomes

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Lentiviral

    Mutation

    Shorter isoform

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid

    #159363

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Tags

    Flag and Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-TetO-hSOX2

    Plasmid

    #206388

    Purpose

    Expresses human SOX2 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid

    #156419

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 SARS-CoV2 TEV-E

    Plasmid

    #154418

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of e…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pDONR223-ZNF770

    Plasmid

    #88562

    Purpose

    Donor Vector containing ZNF770 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF770 (ZNF770 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-45b-HOXD13(+10A)-IDR-mCherry

    Plasmid

    #145282

    Purpose

    Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+10A)-IDR (HOXD13 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-IP-hWIPI4-3×FLAG

    Plasmid

    #215512

    Purpose

    Expresses FLAG tagged human WIPI4.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNOC_dCas9_sgRNA NC

    Plasmid

    #176259

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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