We narrowed to 15,953 results for: grna

1. pXSE901B

   Plasmid

   #91713

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGuide

   Plasmid

   #64711

   Purpose

   Cloning and expression of Streptococcus pyogenes guide RNA

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Streptococcus pyogenes guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6_(GLuc)_INT[3xMS2_SL]

   Plasmid

   #68426

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three MS2 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXCas9H840A

   Plasmid

   #60900

   Purpose

   Dual Expression Vector for Cas9 H840A nickase and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV/3' Box_(GLuc)_TOP1

   Plasmid

   #68434

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide_CjCas9-Puro

   Plasmid

   #202564

   Purpose

   hU6-BsmBI-gRNA-Puro

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   CjCas9 sgRNA cassette
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHEE901

   Plasmid

   #91707

   Purpose

   CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR1068

   Plasmid

   #111092

   Purpose

   BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Mutation

   BlpI site disrupted

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6_(GLuc)_INT[BoBS]

   Plasmid

   #68431

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE6

    Plasmid

    #79476

    Purpose

    sgRNA shuttle vector; module 1E

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEE393

    Plasmid

    #149283

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

12. pYPQ141A2.0

    Plasmid

    #99896

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pG3H-U6SC

    Plasmid

    #134755

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Promoter

    gRNA-U6, zCas9-35SC

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pG3K-U6SC

    Plasmid

    #134754

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Promoter

    gRNA-U6, zCas9-35SC

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEE491

    Plasmid

    #149292

    Purpose

    T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-T Easy-AtpU6-26

    Plasmid

    #160218

    Purpose

    AtpU6-26 Golden Gate level 0 piece to express gRNAs

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtU6-26 promoter
    

    Use

    Golden gate level 0 piece to express grnas

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEE531

    Plasmid

    #149290

    Purpose

    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXFokI-dCas9

    Plasmid

    #60901

    Purpose

    Dual Expression Vector for FokI-dCas9 and gRNA

    Depositor

    Bruce Conklin

    Article

    Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. S9_attL1-EGFP-C-term-attL2

    Plasmid

    #128473

    Purpose

    EGFP entry clone

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    EGFP entry clone
    

    Expression

    Bacterial

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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