pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid
#140003
Purpose
CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
Depositor
Insert
human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
Use
CRISPR; In vitro transcription; t7 promoter
Tags
P2A-EGFP
Expression
Mammalian
Mutation
nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
Promoter
CMV and T7
Available since
March 26, 2020
Availability
Academic Institutions and Nonprofits only
