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Cherry-Myo1b

Plasmid

#187366

Purpose

Expresses rat myosin 1b isoform b domain labelled with Cherry

Depositor

Evelyne Coudrier

Article

Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

Insert

myosin 1b isoform b (Myo1b Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pICH47742::p35S-PE2max-NC::tEURb7

Plasmid

#227693

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).

Use

CRISPR

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pVE13300

Plasmid

#137715

Purpose

Expresses impLbCas12a in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

impLbCas12a

Tags

3xHA, NLS, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pRC0940 (circRNA entry vector)

Plasmid

#189967

Purpose

Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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DHB-mVenus

Plasmid

#136461

Purpose

Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

Depositor

Tobias Meyer, Sabrina Spencer

Article

Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

Insert

DNA Helicase B (HELB Human)

Use

Lentiviral

Tags

mVenus

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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deltaNp63alpha-FLAG

Plasmid

#26979

Depositor

David Sidransky

Article

Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

Insert

ΔNP63alpha (TP63 Human)

Tags

FLAG

Expression

Mammalian

Mutation

dominant negative isoform of p63; aa 1–587

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pJR89

Plasmid

#140096

Purpose

sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

Depositor

Jonathan Weissman

Article

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-miniTurbo-NLS-Blast

Plasmid

#232347

Purpose

Lentiviral control vector expressing miniTurboNLS without dCas9

Depositor

Ali Shilatifard

Article

Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

Insert

miniTurbo

Use

Lentiviral

Tags

3xHA and 3xSV40 NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-Cre

Plasmid

#153207

Purpose

Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

mSncg

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27

Plasmid

#22902

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K27 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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