164,577 results

1. pCDNA3-DCX-TurboID-V5

   Plasmid

   #190740

   Purpose

   Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   doublecortin (DCX Human)
   

   Use

   Tags

   TurboID and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-sgKeap1#1/Cre

   Plasmid

   #173639

   Purpose

   Expresses a Keap1-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Keap1 (Keap1 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX2T-TEV CHMP1B 1-143

   Plasmid

   #108286

   Purpose

   Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-143

   Promoter

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM-TagRFP657

   Plasmid

   #176563

   Purpose

   Expression of TagRFP657 for auxotrophic selection in the absence of methionine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZBTB4-donor

   Plasmid

   #176374

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   ZBTB4 (ZBTB4 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDM326_DPAKa(GBD)-YFP

   Plasmid

   #128701

   Purpose

   expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

   Insert

   GBD domain (aa 731-890) of DPAKa
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP

   Expression

   Mutation

   none

   Promoter

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

   Plasmid

   #111923

   Purpose

   Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_19μ
   

   Use

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, F92W, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pComb3XTT

   Plasmid

   #63891

   Purpose

   Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   TT Fab
   

   Use

   Phage display

   Tags

   6xHis, HA tag, and geneIII

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xHA-miniTurbo-NLS_pCDNA3

   Plasmid

   #107172

   Purpose

   expresses 3xHA-tagged miniTurbo in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Non tagged Avidin

    Plasmid

    #214835

    Purpose

    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Non tagged Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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