We narrowed to 10,123 results for: otos

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CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

Plasmid

#105842

Purpose

Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
Optimized mApple
rtTA3G
SBP Delta-LNGFR

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-GFP HDRT Source (pTR 143)

Plasmid

#112012

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-GFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2018

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Beta

Plasmid

#177962

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )

Expression

Mammalian

Mutation

K417N, E484K, N501Y, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OKMS

Plasmid

#80480

Purpose

piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [10-483]

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OK+9MS

Plasmid

#80482

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [1-483]

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Epsilon

Plasmid

#177964

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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BATF-GFP HDRT Source (pTR 146)

Plasmid

#112015

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

BATF-GFP HDRT (BATF Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-K417N

Plasmid

#177969

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, K417N) (S )

Expression

Mammalian

Mutation

D614G, K417N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N439K

Plasmid

#177970

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N439K) (S )

Expression

Mammalian

Mutation

D614G, N439K

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-L452R

Plasmid

#177971

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, L452R) (S )

Expression

Mammalian

Mutation

D614G, L452R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S477N

Plasmid

#177972

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S477N) (S )

Expression

Mammalian

Mutation

D614G, S477N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N501Y

Plasmid

#177975

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Expression

Mammalian

Mutation

D614G, N501Y

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (JN.1)

Plasmid

#241117

Purpose

Expresses SARS-CoV-2 spike (JN.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (JN.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

ins16MPLF,T19I,R21T,L24del,P25del,P26del,A27S,S50…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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