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We narrowed to 10,347 results for: plasmids 101

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pOTTC588 - pAAV c-fos Nuc-iRFP

Plasmid

#59132

Purpose

An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Infrared Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

mFos

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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ZMYND8-dTAG

Plasmid

#190617

Purpose

Lentiviral expression plasmid of human ZMYND8 fused with FKBP12G36V for dTAG-mediated degradation

Depositor

Junwei Shi

Article

Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

Insert

ZMYND8 (ZMYND8 Human)

Use

Lentiviral

Tags

FKBP12_G36V and Flag, 2xHA

Expression

Mammalian

Mutation

synonymous mutation for sgRNA resistance at aa214…

Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S3-ExRaiAKAR

Plasmid

#160724

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S3-ExRaiAKAR

Use

AAV

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S4-ExRaiCKAR

Plasmid

#160725

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S4-ExRaiCKAR

Use

AAV

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107010

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S2-RAB_EKARev

Plasmid

#160726

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S2-RAB_EKARev

Use

AAV

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CCND2 targeting gRNA

Plasmid

#215318

Purpose

Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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CCND1 targeting gRNA

Plasmid

#215153

Purpose

Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S3-RAB_EKARev

Plasmid

#160727

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S3-RAB_EKARev

Use

AAV

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-HTR6-SNAPf-TRIP

Plasmid

#189617

Purpose

Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6-TRIP_alpha

Use

AAV

Tags

alphaTag in the N-term of HTR6, SNAP-tag in the C…

Expression

Mutation

Promoter

TRE

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC-S227A

Plasmid

#200560

Purpose

Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.CD86.C-HA

Plasmid

#154848

Purpose

Expresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Cluster of Differentiation 86 (Cd86 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2C-ΔC

Plasmid

#200559

Purpose

Secreted mouse CES2C driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

TBG promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

Plasmid

#155194

Purpose

Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated sequence of THBS1 (expresses Amino Acid…

Promoter

CMV

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S1-jRCaMP1a

Plasmid

#160728

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S1-jRCaMP1a

Use

AAV

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-ChR2-YFP

Plasmid

#178725

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_HyPer7_mito

Plasmid

#238968

Purpose

Mammalian neurons HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Mutation

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN002P-M-FLAG-His

Plasmid

#218026

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN002P-M

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN002X-N-FLAG-His

Plasmid

#218033

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN002X-N

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN002W-V-FLAG-His

Plasmid

#218024

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN002W-V

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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