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Plasmid#176281PurposeViral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertmNaChBacMUT-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationE191KPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)
Plasmid#197885PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertGFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)
Plasmid#191145PurposeExpression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.DepositorInsert3UTR(Bdnf)
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut
Plasmid#42046DepositorInsertTNRC6A (TNRC6A Human)
UseTagsFLAG, HA, and SBPExpressionMammalianMutationF951A, F955A, I958A, F960APromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut
Plasmid#42045DepositorInsertTNRC6A (TNRC6A Human)
UseTagsFLAG, HA, and SBPExpressionMammalianMutationR930A, R931A, R932A, R934APromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I affinity mutant (F40-based tension sensor)
Plasmid#119190PurposeThe affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.DepositorInserthuman Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
beta catenin binding mutant of alpha catenin sensor
Plasmid#71711PurposeThis form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctionsDepositorInsertalpha-catenin Δβ-cat mutant FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 48- 163PromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-gp78-FLAG RING mutant C356G H361A
Plasmid#61751Purposeexpress C-terminally FLAG tagged human gp78DepositorInsertgp78 (AMFR Human)
UseTagsFLAGExpressionMammalianMutationC356G H361A; P179L, F262L, Y315C, and D346G (ple…PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant
Plasmid#162020PurposeExpression of GEF dead IQSEC1 mutantDepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationEKPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes 1-480 188/218-mut
Plasmid#158542PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
UseTagsV5ExpressionMammalianMutationAmino acids 1-480, C-terminal deletion, F188D, W2…PromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic
Plasmid#32728DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferase; Luciferase reporter geneTagsExpressionMutation-947 AP-1 binding site TGAGTCA deletionPromoter-962 CCND1 promoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS
Plasmid#112215PurposeTargeted DNA methylationDepositorUseCRISPRTagsmCherryExpressionMutationD10A,H840A,N863A,C710SPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-(935-1026)-NES-mut
Plasmid#42021DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationcontain aa935-1026, F951A, F955A, I958A, F960APromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP
Plasmid#176279PurposeViral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertKir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
UseAAV and Cre/LoxTagsMycExpressionMammalianMutationGYG to AAA (aa144-146)Promoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCP-MU-Luc (MCP-1 mut promoter in pGL3-basic)
Plasmid#40325DepositorInsertMCP-1 promoter (mutated) (Ccl2 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationcontains MCP-1 promoter region through the nucleo…PromoterMCP-1Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut
Plasmid#42040DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa457-532, aa716-772 and aa804-924, F951A…PromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1 SNX9 RYK mutant R286A Y287A K288A
Plasmid#161993PurposeMammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tagDepositorInsertSNX9 (Snx9 Mouse)
UseTagsmCherryExpressionMammalianMutationR286A Y287A K288APromoterAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-24p3/LCN2-282C/EBP mut-LUC
Plasmid#46868Purposemurine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporterDepositorInsert24p3/lipocalin 2 proximal promoter
UseLuciferaseTagsExpressionMutationCEBP site is mutated (see comments)Promoter24p3 promoter (inactive)Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-Dicer-Y926F/R927A (3'-pocket mutant)
Plasmid#51369DepositorInsertDicer (DICER1 Human)
UseTagsFLAGExpressionMammalianMutation3' pocket mutant (Y926F, R927A)PromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-Fyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp
Plasmid#179668PurposeMembrane-bound (Fyn) TEVP-inducible caged-cTVMVPDepositorInsertCaged cTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/myc-His-A-YAP2-1st&2nd WW mut
Plasmid#19061DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsExpressionMammalianMutationTryptophan 199 changed to Alanine,and Proline 202…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)
Plasmid#49061PurposeExpresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationS150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …PromoterCMVAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc
Plasmid#138368PurposeLentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT1-1 (Q867E)-intein-CBD
Plasmid#73301PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationQ867EPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6C-(928-1020)-NES-mut
Plasmid#42027DepositorInsertTNRC6C (TNRC6C Human)
UseTagsEGFP and MycExpressionMammalianMutationcontain aa928-1020, L943A, L947A, M950A, F952APromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT3-1 (C935N)-intein-CBD
Plasmid#73304PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationC935NPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-(925-934)-NLS-mut
Plasmid#42020DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationcontain aa925-934, R930A, R931A, R932A, R934APromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense
Plasmid#46837PurposeExpress cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degradedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
EW152 CMV-TO-Gal4-NZF[Mutated Linkers] (FLP-IN)
Plasmid#236123PurposePlasmid encoding Gal4-NZF transcription factor with deimmunized linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sitesDepositorInsertGal4-NZF (mutated linkers)
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10
Plasmid#219699PurposeLevel 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tagDepositorInsertpSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only