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We narrowed to 6,121 results for: cas9 expression plasmid

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Showing: 1201 - 1230 of 6121 results

  3. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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  5. pCMK

    Plasmid
    #62818
    Purpose
    Bacterial constitutive S. pyogenes Cas9 expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    62818-plasmid-map-sequence-id-114423

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  6. pJAT17

    Plasmid
    #204298
    Purpose
    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EYFP
    Use
    CRISPR
    Promoter
    3XP3
    Available Since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204298-plasmid-map-sequence-id-406176

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  7. pJCHph

    Plasmid
    #185863
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker
    Depositor
    Claudia Vickers
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185863-plasmid-map-sequence-id-370806

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  8. pJCKan

    Plasmid
    #185862
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker
    Depositor
    Claudia Vickers
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185862-plasmid-map-sequence-id-370803

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  9. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  10. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

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  11. pCJ1022

    Plasmid
    #228762
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Pkd2 gRNAs (Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    228762-plasmid-map-sequence-id-455850

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  12. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    pMecp2
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

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  14. lentiCRISPRv2_ZsG gRNA_4

    Plasmid
    #192688
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192688-plasmid-map-sequence-id-379587

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  15. lentiCRISPRv2_ZsG gRNA_1

    Plasmid
    #192685
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192685-plasmid-map-sequence-id-379591

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  16. lentiCRISPRv2_ZsG gRNA_2

    Plasmid
    #192686
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192686-plasmid-map-sequence-id-379590

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  17. lentiCRISPRv2_ZsG gRNA_3

    Plasmid
    #192687
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192687-plasmid-map-sequence-id-379588

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  20. pWS158

    Plasmid
    #90517
    Purpose
    Cas9 gap repair vector - URA3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90517-plasmid-map-sequence-id-287206

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  21. pWS172

    Plasmid
    #90519
    Purpose
    Cas9 gap repair vector - HIS3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    90519-plasmid-map-sequence-id-287208

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  22. pWS171

    Plasmid
    #90518
    Purpose
    Cas9 gap repair vector - LEU2
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90518-plasmid-map-sequence-id-288271

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  23. pWS175

    Plasmid
    #90962
    Purpose
    Cas9 gap repair vector - HygR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90962-plasmid-map-sequence-id-287209

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  24. pWS174

    Plasmid
    #90961
    Purpose
    Cas9 gap repair vector - NatR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    90961-plasmid-map-sequence-id-207608

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  25. pWS176

    Plasmid
    #90963
    Purpose
    Cas9 gap repair vector - ZeoR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    90963-plasmid-map-sequence-id-342430

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  26. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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  27. pITI1

    Plasmid
    #166094
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166094-plasmid-map-sequence-id-329610

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  28. pJAT57

    Plasmid
    #204303
    Purpose
    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Promoter
    MHC
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204303-plasmid-map-sequence-id-405488

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  29. PB-Puro-U6-gRNA-pA plasmid

    Plasmid
    #247671
    Purpose
    spCas9 gRNA is driven by human U6 promoter, with the gRNA cassette between PuroR and pA, making it detectable in polyA-based RNA-seq.
    Depositor
    Tae Hyun Kim
    Insert
    Puromycin resistant gene
    Expression
    Mammalian
    Promoter
    TK promoter
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247671-plasmid-map-sequence-id-491686

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  30. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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Showing: 1201 - 1230 of 6121 results