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We narrowed to 6,164 results for: cas9 expression plasmid

Showing: 1201 - 1230 of 6164 results

  1. pMZ377

    Plasmid
    #74214
    Purpose
    Combined sgRNA/Cas9 vector with LEU2 marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74214-plasmid-map-sequence-id-141045

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  2. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  3. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

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  4. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

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  5. pCJ1022

    Plasmid
    #228762
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Pkd2 gRNAs (Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    228762-plasmid-map-sequence-id-455850

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  6. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid
    #154429
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Depositor
    David Liu
    Article
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154429-plasmid-map-sequence-id-304585

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  7. lentiCRISPRv2_ZsG gRNA_4

    Plasmid
    #192688
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #4
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192688-plasmid-map-sequence-id-379587

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  8. lentiCRISPRv2_ZsG gRNA_1

    Plasmid
    #192685
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192685-plasmid-map-sequence-id-379591

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  9. lentiCRISPRv2_ZsG gRNA_2

    Plasmid
    #192686
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192686-plasmid-map-sequence-id-379590

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  10. lentiCRISPRv2_ZsG gRNA_3

    Plasmid
    #192687
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192687-plasmid-map-sequence-id-379588

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  11. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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  12. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    pMecp2
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

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  13. pWS158

    Plasmid
    #90517
    Purpose
    Cas9 gap repair vector - URA3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90517-plasmid-map-sequence-id-287206

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  14. pWS175

    Plasmid
    #90962
    Purpose
    Cas9 gap repair vector - HygR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90962-plasmid-map-sequence-id-287209

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  15. pWS172

    Plasmid
    #90519
    Purpose
    Cas9 gap repair vector - HIS3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    90519-plasmid-map-sequence-id-287208

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  16. pWS174

    Plasmid
    #90961
    Purpose
    Cas9 gap repair vector - NatR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    90961-plasmid-map-sequence-id-207608

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  17. pWS171

    Plasmid
    #90518
    Purpose
    Cas9 gap repair vector - LEU2
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90518-plasmid-map-sequence-id-288271

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  18. pWS176

    Plasmid
    #90963
    Purpose
    Cas9 gap repair vector - ZeoR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    90963-plasmid-map-sequence-id-342430

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  19. pJAT57

    Plasmid
    #204303
    Purpose
    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204303-plasmid-map-sequence-id-405488

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  20. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  21. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  22. pWS173

    Plasmid
    #90960
    Purpose
    Cas9 gap repair vector - KanR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90960-plasmid-map-sequence-id-261712

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  23. pUDP082

    Plasmid
    #103875
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103875-plasmid-map-sequence-id-199262

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  24. pUDP025

    Plasmid
    #103874
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103874-plasmid-map-sequence-id-199263

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  25. pUDP013

    Plasmid
    #103873
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103873-plasmid-map-sequence-id-199239

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  27. pAAV-dSa-VPR

    Plasmid
    #99651
    Purpose
    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VP64-p65-RTA activator
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    99651-plasmid-map-sequence-id-191797

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  28. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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  29. CRISPR-Sa-TdTom. Reporter

    Plasmid
    #99652
    Purpose
    Red fluorescent reporter that can be activated by dSa Cas9 activator indicating activity
    Depositor
    George Church
    Insert
    RFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    99652-plasmid-map-sequence-id-192053

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  30. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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Showing: 1201 - 1230 of 6164 results