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pXPR_124
Plasmid#133461PurposeEF1a promoter expresses SpyoCas9 and P2A provides EGFPDepositorInsertSpyoCas9
UseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF221
Plasmid#121669PurposeU6-sgRNA EF1a-mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-GpNLuc
Plasmid#135935PurposeExpresses the GpNLuc LumiFluor in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sico-Red
Plasmid#84882PurposeCre-inducible shRNADepositorInsertSico-Red
UseAAVTagsmCherryExpressionMutationPromoterEF1a and CMVAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
RAB14 S25N
Plasmid#61495Purposeto overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoterDepositorInsertRAB14 (RAB14 Human)
UseLentiviralTagsExpressionMutationS25N, inactive formPromoterEF1aAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-ReaChR-P2A-dTomato
Plasmid#114368Purposeexpresses a Cre-dependent red light-shifted channelrhodopsin variantDepositorInsertReaChR-P2A-dTomato
UseAAVTagsdTomatoExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB14 Q70L
Plasmid#61494Purposeto overexpress constitutively active RAB14. Vector includes GFP driven by hUbC promoterDepositorInsertRAB14 (RAB14 Human)
UseLentiviralTagsExpressionMutationQ70L, active formPromoterEF1aAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_zeo insert c-MYC
Plasmid#164636PurposesgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64DepositorInserttarget sequence for c-Myc activation (MYC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-DIO-CB2-EGFP
Plasmid#91946PurposeExpression of mouse CB2 cannabinoid receptors and eGFP in the presence of CreDepositorInsertsEGFP
type-2 cannabinoid receptor
UseAAVTagsFLAG and NoneExpressionMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-GFP
Plasmid#170176PurposeExpresses GFP under the control of EF1a short promoterDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptwB
Plasmid#48735PurposeTandem dual tomato plasmidDepositorInsertTandem dual tomato
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-OgNLuc
Plasmid#135936PurposeExpresses the OgNLuc LumiFluor in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR1-EYFP
Plasmid#114370Purposeexpresses Flpo recombinase-dependent GtACR1DepositorInsertGtACR1-EYFP (NEWENTRY )
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV.pCry1(408).DIO.Luciferase
Plasmid#192623PurposeCre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoterDepositorInsertluciferase
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAID-CMV linearizing in pX330
Plasmid#140609PurposeCrispr/Cas9 plasmid for linearization of pAID-CMVDepositorInsertsgRNA for pAID-EF1a plasmid digestion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF:iCas9
Plasmid#138267PurposeExpression of mTN3-GGS6-dCas9 (iCas9) in human.DepositorInsertmTN3-GGS6-dCas9
UseCRISPRTagsExpressionMammalianMutationCas9-D10A, H840APromoterEf1aAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF‐PIK3IP1
Plasmid#49214Purposeexpresses PIK3IP1 in mammalian cellsDepositorInsertPIK3IP1 (Pik3ip1 Mouse)
UseTags6X-his and mycExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCF713
Plasmid#121665PurposeU6-sgRNA EF1a-ProCas9-FlaviS6-P2A-PuroDepositorInsertsUseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
IDP432
Plasmid#87000Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertSNAP-Nlg1AB-1xλN(i)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP431
Plasmid#86999Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertCLIP-Nrx1B-4xλN(i)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP455
Plasmid#87001Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertbarcode landing site Gfp-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB1: All in one SpCas9 expression and sgRNA cloning backbone
Plasmid#228363PurposemU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherryDepositorTypeEmpty backboneUseCRISPR and Lentiviral; Spcas9 nuclease and spcas9…TagsExpressionMammalianMutationPromotermU6Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
MCP-YPet
Plasmid#215747PurposeExpresses MS2 coat protein (MCP) fused to YPetDepositorInsertYPet
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
IDP254
Plasmid#87004Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpostCLAP
UseSynthetic BiologyTagsspGFP (1-10)ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP253
Plasmid#87003Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpreCLAP
UseSynthetic BiologyTagsGFP11ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP379
Plasmid#87006Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpostLib-mRFP-4xboxB
UseSynthetic BiologyTagsmRFPExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDP378
Plasmid#87005Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpreLib-bfp-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SYP-HRP
Plasmid#117185PurposePlasmid name in publication: pAAV-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertSYP-HRP
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NGN2-EMX1 BSD-mApple
Plasmid#182311PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mAppleUseTagsT2A-mycNLS-mAppleExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF.DEST51-mScarlet
Plasmid#207960PurposeVector for overexpression of the mScarlet fluorescent proteinDepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-HA
Plasmid#188521PurposeEncodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsHAExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-dAPEX2
Plasmid#117174PurposePeroxidase reporter for multiplexed electron microscopy labelingDepositorInsertdAPEX2
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-tmpknot-epegRNA-puro backbone
Plasmid#214089PurposeLentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-pegRNA-puro backbone
Plasmid#214085PurposeLentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 KRAB
Plasmid#188499PurposeExpresses FLAG tagged SpdCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-miniTurbo-NLS-Blast
Plasmid#232347PurposeLentiviral control vector expressing miniTurboNLS without dCas9DepositorInsertminiTurbo
UseLentiviralTags3xHA and 3xSV40 NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-Cas9
Plasmid#96924Purposefor Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111DepositorInsertCas9
UseLentiviralTags3xFlag and V5ExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-KRAB-dCas9
Plasmid#96918Purposefor CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121DepositorInsertdCas9
UseLentiviralTagsKRABExpressionMammalianMutationdCas9PromoterEF1aAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF-BFP
Plasmid#138272PurposeExpression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editorDepositorInsertBFP H66 PAM
UseCRISPRTagsExpressionMammalianMutationH66, PAM motifPromoterEf1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
phMYT1L-N174
Plasmid#66809Purpose2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistanceDepositorInsertMYT1L (MYT1L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 VPR
Plasmid#188509PurposeExpresses FLAG tagged SpdCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIV-iRFP720-E2A-Luc
Plasmid#104587PurposeLentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoterDepositorInsertiRFP720-E2A-Luciferase
UseLentiviral and LuciferaseTagsbi-cistronicExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-mTagBFP2
Plasmid#216126PurposeLentiviral vector for expressing mTagBFP2.DepositorInsertEF1a-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR V5-RNF25_IRES-mCherry
Plasmid#198386PurposeRNF25 expressionDepositorInsertRNF25 (RNF25 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SaCas9
Plasmid#188494PurposeExpresses FLAG tagged SaCas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_MCP-LSD1_Hygro
Plasmid#138457Purposelenti vector encoding the MS2-LSD1 with a 2A Hygro resistance markerDepositorInsertLSD1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDA_118
Plasmid#133459PurposeU6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrRDepositorInsertcontrol guide
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
UseTagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only
